GNOM/FEWER ROOTS is Required for the Establishment of an Auxin Response Maximum for Arabidopsis Lateral Root Initiation
| العنوان: | GNOM/FEWER ROOTS is Required for the Establishment of an Auxin Response Maximum for Arabidopsis Lateral Root Initiation |
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| المؤلفون: | Yumiko Takebayashi, Hiroyuki Kasahara, Yuji Kamiya, Tetsuro Mimura, Hidehiro Fukaki, Koichi Toyokura, Ken-ichi Okumura, Tatsuaki Goh |
| المصدر: | Plant and Cell Physiology |
| بيانات النشر: | Oxford University Press, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Auxin efflux, Arabidopsis thaliana, Physiology, Mutant, Green Fluorescent Proteins, Molecular Sequence Data, Arabidopsis, Mutation, Missense, Genes, Recessive, Plant Science, Plant Roots, Plant Growth Regulators, Auxin, Gene Expression Regulation, Plant, Xylem, Guanine Nucleotide Exchange Factors, Amino Acid Sequence, Special Focus Issue – Regular Papers, Lateral root formation, Alleles, chemistry.chemical_classification, Genetics, Protein Synthesis Inhibitors, Brefeldin A, biology, Dose-Response Relationship, Drug, Indoleacetic Acids, Arabidopsis Proteins, Lateral root, fungi, Wild type, food and beverages, Membrane Transport Proteins, Cell Biology, General Medicine, biology.organism_classification, Cell biology, Phenotype, chemistry, Seedlings, GNOM, Sequence Alignment |
| الوصف: | Lateral root (LR) formation in vascular plants is regulated by auxin. The mechanisms of LR formation are not fully understood. Here, we have identified a novel recessive mutation in Arabidopsis thaliana, named fewer roots (fwr), that drastically reduces the number of LRs. Expression analyses of DR5::GUS, an auxin response reporter, and pLBD16::GUS, an LR initiation marker, suggested that FWR is necessary for the establishment of an auxin response maximum in LR initiation sites. We further identified that the fwr phenotypes are caused by a missense mutation in the GNOM gene, encoding an Arf-GEF (ADP ribosylation factor-GDP/GTP exchange factor), which regulates the recycling of PINs, the auxin efflux carriers. The fwr roots showed enhanced sensitivity to brefeldin A in a root growth inhibition assay, indicating that the fwr mutation reduces the Arf-GEF activity of GNOM. However, the other developmental processes except for LR formation appeared to be unaffected in the fwr mutant, indicating that fwr is a weaker allele of gnom compared with the other gnom alleles with pleiotropic phenotypes. The localization of PIN1-green fluorescent protein (GFP) appeared to be unaffected in the fwr roots but the levels of endogenous IAA were actually higher in the fwr roots than in the wild type. These results indicate that LR initiation is one of the most sensitive processes among GNOM-dependent developmental processes, strongly suggesting that GNOM is required for the establishment of the auxin response maximum for LR initiation, probably through the regulation of local and global auxin distribution in the root. |
| اللغة: | English |
| تدمد: | 1471-9053 0032-0781 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b87c200a62f4b1e2e18927defd76d00eTest http://europepmc.org/articles/PMC3589829Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b87c200a62f4b1e2e18927defd76d00e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14719053 00320781 |
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