A high-throughput DNA methylation analysis of a single cell
| العنوان: | A high-throughput DNA methylation analysis of a single cell |
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| المؤلفون: | Joachim W. Ellwart, Axel Schumacher, Petra Hartmann, Roland Kirchner, Martin Kantlehner, Marianna Alunni-Fabbroni |
| المصدر: | Nucleic Acids Research Nucleic Acids Res. 39, e44:e44 (2011) |
| بيانات النشر: | Oxford University Press, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Biology, Polymerase Chain Reaction, law.invention, Cell Line, chemistry.chemical_compound, Single-cell analysis, law, Cell Line, Tumor, Genetics, Humans, Epigenetics, Lymphocytes, Gene, Human cancers, Stem-cells, In-vitro, Lines, Expression, Hypermethylation, Patterns, Promoter, Embryos, Polymerase chain reaction, Methylation, DNA Restriction Enzymes, DNA Methylation, High-Throughput Screening Assays, chemistry, CpG site, DNA methylation, Methods Online, CpG Islands, Single-Cell Analysis, DNA |
| الوصف: | In recent years, the field of epigenetics has grown dramatically and has become one of the most dynamic and fast-growing branches of molecular biology. The amount of diseases suspected of being influenced by DNA methylation is rising steadily and includes common diseases such as schizophrenia, bipolar disorder, Alzheimer's disease, diabetes, atherosclerosis, cancer, major psychosis, lupus and Parkinson's disease. Due to cellular heterogeneity of methylation patterns, epigenetic analyses of single cells become a necessity. One rationale is that DNA methylation profiles are highly variable across individual cells, even in the same organ, dependent on the function of the gene, disease state, exposure to environmental factors (e.g. radiation, drugs or nutrition), stochastic fluctuations and various other causes. Using a polymerase chain reaction (PCR)-slide microreaction system, we present here a methylation-sensitive PCR analysis, the restriction enzyme-based single-cell methylation assay (RSMA), in the analysis of DNA methylation patterns in single cells. This method addresses the problems of cell heterogeneity in epigenetics research; it is comparably affordable, avoids complicated microfluidic systems and offers the opportunity for high-throughput screening, as many single cells can be screened in parallel. In addition to this study, critical principles and caveats of single cell methylation analyses are discussed. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1362-4962 0305-1048 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::397dacdac690b528a4f9ead4c972ab04Test http://europepmc.org/articles/PMC3074158Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....397dacdac690b528a4f9ead4c972ab04 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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