Sensory Response of Transplanted Astrocytes in Adult Mammalian Cortex In Vivo
| العنوان: | Sensory Response of Transplanted Astrocytes in Adult Mammalian Cortex In Vivo |
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| المؤلفون: | Xiaowei Chen, Chunhai Chen, Zhuan Zhou, Zhou Zhou, Jingcheng Li, Liting Wang, Zhiqi Yang, Wei Sun, Ping Deng, Kuan Zhang, Zhengping Yu, Meng Wang, Qinlong Ma, Xiang Liao, Lianghong Zheng, Li Mingli, Wenjing He, Hongbo Jia, Jian Lu |
| المصدر: | Cerebral Cortex (New York, NY) |
| بيانات النشر: | Oxford University Press, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, somatosensory cortex, Cognitive Neuroscience, Action Potentials, Sensory system, Neocortex, Biology, Somatosensory system, 03 medical and health sciences, Cellular and Molecular Neuroscience, Mice, 0302 clinical medicine, Neural Stem Cells, Cortex (anatomy), medicine, Animals, Humans, Sensory cortex, Calcium Signaling, glial precursor transplantation, astrocytes, Cell Differentiation, Original Articles, two-photon Ca2+ imaging, Neural stem cell, Electric Stimulation, Transplantation, 030104 developmental biology, medicine.anatomical_structure, Female, sensory response, Neuroscience, Neural development, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: | Glial precursor transplantation provides a potential therapy for brain disorders. Before its clinical application, experimental evidence needs to indicate that engrafted glial cells are functionally incorporated into the existing circuits and become essential partners of neurons for executing fundamental brain functions. While previous experiments supporting for their functional integration have been obtained under in vitro conditions using slice preparations, in vivo evidence for such integration is still lacking. Here, we utilized in vivo two-photon Ca(2+) imaging along with immunohistochemistry, fluorescent indicator labeling-based axon tracing and correlated light/electron microscopy to analyze the profiles and the functional status of glial precursor cell-derived astrocytes in adult mouse neocortex. We show that after being transplanted into somatosensory cortex, precursor-derived astrocytes are able to survive for more than a year and respond with Ca(2+) signals to sensory stimulation. These sensory-evoked responses are mediated by functionally-expressed nicotinic receptors and newly-established synaptic contacts with the host cholinergic afferents. Our results provide in vivo evidence for a functional integration of transplanted astrocytes into adult mammalian neocortex, representing a proof-of-principle for sensory cortex remodeling through addition of essential neural elements. Moreover, we provide strong support for the use of glial precursor transplantation to understand glia-related neural development in vivo. |
| اللغة: | English |
| تدمد: | 1460-2199 1047-3211 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c5d4595e7bb1180a2ee639ff681b100Test http://europepmc.org/articles/PMC5004757Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2c5d4595e7bb1180a2ee639ff681b100 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602199 10473211 |
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