Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF–DDX19B interaction
| العنوان: | Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF–DDX19B interaction |
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| المؤلفون: | Kwon Jeong, Leehyeon Kim, Hyun Jung Hwang, Joori Park, Oliver Mühlemann, Simone C. Rufener, Hyun Kyu Song, Yeonkyoung Park, Yoon Ki Kim |
| المصدر: | Nucleic Acids Research Park, Yeonkyoung; Park, Joori; Hwang, Hyun Jung; Kim, Leehyeon; Jeong, Kwon; Song, Hyun Kyu; Rufener, Simone C.; Mühlemann, Oliver; Kim, Yoon Ki (2021). Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF–DDX19B interaction. Nucleic acids research, 49(14), pp. 8261-8276. Oxford University Press 10.1093/nar/gkab579 <http://dx.doi.org/10.1093/nar/gkab579Test> |
| بيانات النشر: | Oxford University Press, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cytoplasm, Nucleocytoplasmic Transport Proteins, AcademicSubjects/SCI00010, Biology, DEAD-box RNA Helicases, 03 medical and health sciences, 0302 clinical medicine, Polysome, 540 Chemistry, Genetics, Initiation factor, Humans, Protein Interaction Maps, RNA, Messenger, Nuclear pore, Eukaryotic Initiation Factors, Molecular Biology, Nuclear Cap-Binding Protein Complex, 030304 developmental biology, 0303 health sciences, Messenger RNA, Cap binding complex, Translation (biology), mRNA surveillance, Cell biology, Nonsense Mediated mRNA Decay, RNA Cap-Binding Proteins, Protein Biosynthesis, 570 Life sciences, biology, 030217 neurology & neurosurgery, HeLa Cells |
| الوصف: | Newly synthesized mRNA is translated during its export through the nuclear pore complex, when its 5′-cap structure is still bound by the nuclear cap-binding complex (CBC), a heterodimer of cap-binding protein (CBP) 80 and CBP20. Despite its critical role in mRNA surveillance, the mechanism by which CBC-dependent translation (CT) is regulated remains unknown. Here, we demonstrate that the CT initiation factor (CTIF) is tethered in a translationally incompetent manner to the perinuclear region by the DEAD-box helicase 19B (DDX19B). DDX19B hands over CTIF to CBP80, which is associated with the 5′-cap of a newly exported mRNA. The resulting CBP80–CTIF complex then initiates CT in the perinuclear region. We also show that impeding the interaction between CTIF and DDX19B leads to uncontrolled CT throughout the cytosol, consequently dysregulating nonsense-mediated mRNA decay. Altogether, our data provide molecular evidence supporting the importance of tight control of local translation in the perinuclear region. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1362-4962 0305-1048 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::305a87f55891849e07a51edab2029f25Test http://europepmc.org/articles/PMC8373075Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....305a87f55891849e07a51edab2029f25 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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