دورية أكاديمية
Whole-body scanning PCR; a sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides
| العنوان: | Whole-body scanning PCR; a sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides |
|---|---|
| المؤلفون: | Boos, Julien, Kirk, David William, Dattler, Andre, Piccolotto, Mari-Luz, Zuercher, Werner, Gfeller, Sandro, Neuner, Philippe, Christensen, Jesper, Wishart, William L., Beverly, Michael, Von Arx, Fabian, Litherland, Karine, Van De Kerkhof, Esther, Swart, Pieter Jacob, Faller, Thomas, Beyerbach, Armin, Morrissey, David, Hunziker, Juerg, Beuvink, Iwan |
| بيانات النشر: | Oxford Journals |
| سنة النشر: | 2012 |
| المجموعة: | The Novartis Repository (Novartis, Switzerland) |
| الوصف: | Efficient tissue-specific delivery is a crucial factor in the successful development of therapeutic oligonucleotides. Screening for novel delivery methods with unique tissue-homing properties requires a rapid, sensitive, flexible and unbiased technique able to visualize the in vivo biodistribution of these oligonucleotides. Here we present whole body scanning PCR, a platform that relies on the local extraction of tissues from a mouse whole body section followed by the conversion of target-specific qPCR signals into an image. This platform was designed to be compatible with a novel RTqPCR assay for the detection of siRNAs and with an assay suitable for the detection of heavily chemically modified oligonucleotides which we termed Chemical-Ligation qPCR (CL-qPCR). In addition to this, the platform can also be used to investigate the global expression of endogenous mRNAs and non-coding RNAs. Incorporation of other detection systems, such as aptamers, could even further expand the use of this technology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | Boos, Julien, Kirk, David William, Dattler, Andre, Piccolotto, Mari-Luz, Zuercher, Werner, Gfeller, Sandro, Neuner, Philippe, Christensen, Jesper, Wishart, William L., Beverly, Michael, Von Arx, Fabian, Litherland, Karine, Van De Kerkhof, Esther, Swart, Pieter Jacob, Faller, Thomas, Beyerbach, Armin, Morrissey, David, Hunziker, Juerg and Beuvink, Iwan (2012) Whole-body scanning PCR; a sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides. Nucleic Acids Research, Nuclei. ISSN 0305-1048; PMID: 23766292; doi:10.1093/nar/gkt515 |
| الإتاحة: | https://doi.org/10.1093/nar/gkt515Test https://oak.novartis.com/7984Test/ http://nar.oxfordjournals.org/content/early/2013/06/13/nar.gkt515.longTest |
| رقم الانضمام: | edsbas.FFBE2608 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |