Abstract P3091: Transgenic Expression Of Nrf2 Induces A Pro-reductive Stress And Adaptive Cardiac Remodeling In Mice
| العنوان: | Abstract P3091: Transgenic Expression Of Nrf2 Induces A Pro-reductive Stress And Adaptive Cardiac Remodeling In Mice |
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| المؤلفون: | Arun Jyothidasan, Saravanakumar Murugesan, Justin Quiles, Anil Kumar Challa, Senthil Kumar Cinghu, Vivek Nanda, Rajasekaran N Namakkal-Soorappan |
| المصدر: | Circulation Research. 131 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Physiology, Cardiology and Cardiovascular Medicine |
| الوصف: | Background: NFE2L2, nuclear factor, erythroid 2 like 2 (Nrf2), is a transcription factor that protects cells through maintaining a homeostatic redox state during stress. Constitutive expression of Nrf2 (CaNrf2-TG) was previously shown to be pathological to the heart over time. We tested a hypothesis that the cardiac-specific expression of full length Nrf2 (mNrf2-TG) would moderately increase the basal antioxidant defense, triggering a pro-reductive environment leading to adaptive cardiac remodeling. Methods: Cardiac-specific full-length Nrf2 transgenic (mNrf2-TG) and non-transgenic (NTG) mice hearts on the C57/BL6 background at 7-8 months of age were used to analyze the transcriptome and cardiac structure/function. Next generation sequencing (NGS) for RNA profiling (n=3/group) and qPCR-based validation (n=5/group) of the NGS data were accomplished. Myocardial redox levels and imaging (echocardiography) were performed. Results: Transcriptomic analysis revealed that out of 14665 identified mRNAs, 679 were differently expressed (DEG) in TG hearts. Of 679 DEGs, 429 were upregulated and 251 were downregulated significantly (FC>2, P Conclusion: The effects of excess Nrf2-driven antioxidant transcriptome revealed a pro-reductive condition in the myocardium leading to an adaptive cardiac remodeling. While pre-conditioning the myocardial redox with excess antioxidants (i.e. pro-reductive state) could be beneficial against oxidative stress, a chronic pro-reductive environment in the myocardium might transition the adaptation to pathological remodeling. |
| تدمد: | 1524-4571 0009-7330 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::3d7f09a1b52388eb2291dbca1dc1f2e3Test https://doi.org/10.1161/res.131.suppl_1.p3091Test |
| رقم الانضمام: | edsair.doi...........3d7f09a1b52388eb2291dbca1dc1f2e3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244571 00097330 |
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