Nonbone Marrow-Derived Circulating Progenitor Cells Contribute to Postnatal Neovascularization Following Tissue Ischemia
| العنوان: | Nonbone Marrow-Derived Circulating Progenitor Cells Contribute to Postnatal Neovascularization Following Tissue Ischemia |
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| المؤلفون: | Fred Fändrich, Alexandra Aicher, Joachim W. Ellwart, Stefanie Dimmeler, Christopher Heeschen, Ken-ichiro Sasaki, John P. Cooke, Reiner Siebert, Markus Rentsch |
| المصدر: | Circulation Research. 100:581-589 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Physiology, Angiogenesis, Ischemia, Hindlimb, Biology, medicine.disease, Small intestine, Neovascularization, Vasculogenesis, medicine.anatomical_structure, Immunology, medicine, Progenitor cell, Stem cell, medicine.symptom, Cardiology and Cardiovascular Medicine |
| الوصف: | Circulating progenitor cells home to sites of postnatal neovascularization and differentiate into endothelial cells but questions remain regarding the source of these cells. Indeed, a recent study suggests that nonbone marrow-derived cells may be even more important than bone marrow-derived cells in the setting of transplant arteriosclerosis. Thus, we aimed to thoroughly investigate the contribution of nonbone marrow-derived progenitor cells for neovascularization. We exclusively identified nonbone marrow-derived progenitor cells by combining a parabiosis model with reverse bone marrow transplantation followed by hindlimb ischemia. In this model, nonbone marrow-derived circulating progenitor cells attributed for 74±13% of the circulating progenitor cells that incorporated into the ischemic hindlimb. Increasing evidence suggests that organs such as small intestine and liver contain a considerable number of tissue resident progenitor cells and, thus, represent putative sources for nonbone marrow-derived progenitors. To track organ-derived progenitors, we transplanted sex-mismatched small intestine or liver, respectively, into rats followed by induction of hindlimb ischemia. These experiments show that organ-derived progenitor cells are contributing to postnatal vasculogenesis (intestine: 4.7±3.7%; liver: 6.3±2.2%). Based on the subsequent observation that liver-derived nonhematopoietic c-kit + CD45 − progenitors are mobilized on induction of hindlimb ischemia, we prospectively isolated and intravenously infused these progenitors from murine livers. The isolated cells demonstrated a marked capacity for enhancing neovascularization and restoring blood flow to the ischemic hindlimb (no cells: 26.4±4.8% of normal blood flow; c-kit + CD45 − cells: 67.0±8.0% of normal flow; P + CD45 − progenitors contribute to postnatal neovascularization to an extent that is similar to that of bone marrow-derived progenitor cells. Intestine and liver represent a rich source for mobilized tissue-residing progenitor cells. |
| تدمد: | 1524-4571 0009-7330 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::144dcab2db985701a0356a1e145ce33fTest https://doi.org/10.1161/01.res.0000259562.63718.35Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........144dcab2db985701a0356a1e145ce33f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244571 00097330 |
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