Multiple noncoding exons 1 of nuclear receptors NR4A family (nerve growth factor-induced clone B, Nur-related factor 1 and neuron-derived orphan receptor 1) and NR5A1 (steroidogenic factor 1) in human cardiovascular and adrenal tissues
| العنوان: | Multiple noncoding exons 1 of nuclear receptors NR4A family (nerve growth factor-induced clone B, Nur-related factor 1 and neuron-derived orphan receptor 1) and NR5A1 (steroidogenic factor 1) in human cardiovascular and adrenal tissues |
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| المؤلفون: | Katsuharu Uchiyama, Hidekazu Ino, Masashi Oe, Shunsuke Mori, Yuji Maeda, Takashi Yoneda, Toshinari Tsubokawa, Yasuhiro Nakamura, Masashi Demura, Yoshiyu Takeda, Hironobu Sasano, Yoshiki Demura, Shigehiro Karashima, Noboru Fujino, Mitsuhiro Kometani, Toshitaka Sawamura, Masakazu Yamagishi, Yuan Cheng, Katsuhiko Ono, Fen Wang, Mikio Namiki |
| المصدر: | Journal of Hypertension. 29:1185-1195 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Steroidogenic factor 1, endocrine system, medicine.medical_specialty, cardiac muscle, adrenal cortex, Physiology, Blotting, Western, Molecular Sequence Data, Receptors, Cytoplasmic and Nuclear, Biology, chemistry.chemical_compound, Internal medicine, Adrenal Glands, Internal Medicine, medicine, Humans, nuclear receptor, Cells, Cultured, DNA Primers, Orphan receptor, noncoding exon, Aldosterone, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Adrenal cortex, Adrenal gland, Myocardium, artery, DNA, Exons, aldosterone-producing adenoma, Angiotensin II, Neuron-derived orphan receptor 1, CYP11B2, Endocrinology, medicine.anatomical_structure, Nuclear receptor, chemistry, Cardiology and Cardiovascular Medicine |
| الوصف: | 金沢大学医薬保健研究域医学系 Objective: Nuclear receptors are involved in a wide variety of functions, including aldosteronogenesis. Nuclear receptor families NR4A [nerve growth factor-induced clone B (NGFIB), Nur-related factor 1 (NURR1) and neuron-derived orphan receptor 1 (NOR1)] and NR2F [chicken ovalbumin upstream promoter-transcription factor 1 (COUP-TFI), COUP-TFII and NR2F6) activate, whereas NR5A1 [steroidogenic factor 1 (SF1)] represses CYP11B2 (aldosterone synthase) gene transcription. The present study was undertaken to elucidate the mechanism of differential regulation of nuclear receptors between cardiovascular and adrenal tissues. Methods: We collected tissues of artery (n = 9), cardiomyopathy muscle (n = 9), heart muscle (noncardiomyopathy) (n = 6), adrenal gland (n = 9) and aldosterone-producing adenoma (APA) (n = 9). 5′-rapid amplification of cDNA ends (RACE) identified transcription start sites. Multiplex reverse-transcription PCR (RT-PCR) determined use of alternative noncoding exons 1 (ANEs). Results: In adrenocortical H295R cells, angiotensin II, KCl or cAMP, all stimulated CYP11B2 transcription and NR4A was upregulated, whereas NR2F and NR5A1 were downregulated. 5′-RACE and RT-PCR revealed four ANEs of NGFIB (NR4A1), three of NURR1 (NR4A2), two of NOR1 (NR4A3) and two of SF1 (NR5A1) in cardiovascular and adrenal tissues. Quantitative multiplex RT-PCR showed NR4A and NR5A1 differentially employed multiple ANEs in a tissue-specific manner. The use of ANEs of NGFIB and NURR1 was significantly different between APA and artery. Changes in use of ANEs of NGFIB and NOR1 were observed between cardiomyopathy and noncardiomyopathy. The NR4A mRNA levels in artery were high compared with cardiac and adrenal tissues, whereas the NR5A1 mRNA level in adrenal tissues was extremely high compared with cardiovascular tissues. Conclusion: NR4A and NR5A1 genes are complex in terms of alternative promoter use. The use of ANEs may be associated with the pathophysiology of the heart and adrenal gland. © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins. |
| تدمد: | 0263-6352 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f45afbf1ae2fb034a3a4130fc4a5621cTest https://doi.org/10.1097/hjh.0b013e32834626bbTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f45afbf1ae2fb034a3a4130fc4a5621c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02636352 |
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