Measurement of Pancreatic Polypeptide ans Its Peptide Variant in Human Serum and Plasma by Immunocapture-Liquid-Chromatography-Tandem Mass Spectrometry. Reference Intervals and Practical Assay Considerations
التفاصيل البيبلوغرافية
العنوان:
Measurement of Pancreatic Polypeptide ans Its Peptide Variant in Human Serum and Plasma by Immunocapture-Liquid-Chromatography-Tandem Mass Spectrometry. Reference Intervals and Practical Assay Considerations
Pancreatic polypeptide (PP) is secreted by pancreatic islets after ingestion of food. Meal composition is associated with concentration of PP released in circulation. PP can be elevated in blood of patients with endocrine pancreatic tumors, supporting its use as a biomarker. We developed an Immunocapture-Liquid Chromatography- Tandem Mass Spectrometry (IC-LC-MS/MS) assay to quantify Pancreatic Polypeptide (PP) and its metabolite, PP3-36, in human serum or plasma. PP and PP3-36 were enriched from serum or plasma using immunopurification and the samples were analyzed using LC-MS/MS (AB SCIEX 5500) Total imprecision of the method was less than 20%; LOD and LOQ for PP and PP3-36 were 5 pg/mL and 10 pg/mL, respectively. Reference intervals in healthy subjects were
