دورية أكاديمية
Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells
| العنوان: | Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells |
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| المؤلفون: | Semrau, Stefan, Goldmann, Johanna E., Soumillon, Magali, Mikkelsen, Tarjei S., Jaenisch, Rudolf, van Oudenaarden, Alexander |
| بيانات النشر: | Nature Publishing Group UK |
| سنة النشر: | 2017 |
| المجموعة: | Harvard University: DASH - Digital Access to Scholarship at Harvard |
| الوصف: | Gene expression heterogeneity in the pluripotent state of mouse embryonic stem cells (mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and lineage commitment have not been studied systematically at the single-cell level. Here we measure the gene expression dynamics of retinoic acid driven mESC differentiation from pluripotency to lineage commitment, using an unbiased single-cell transcriptomics approach. We find that the exit from pluripotency marks the start of a lineage transition as well as a transient phase of increased susceptibility to lineage specifying signals. Our study reveals several transcriptional signatures of this phase, including a sharp increase of gene expression variability and sequential expression of two classes of transcriptional regulators. In summary, we provide a comprehensive analysis of the exit from pluripotency and lineage commitment at the single cell level, a potential stepping stone to improved lineage manipulation through timing of differentiation cues. ; Version of Record |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653659/pdfTest/; Nature Communications; Semrau, Stefan, Johanna E. Goldmann, Magali Soumillon, Tarjei S. Mikkelsen, Rudolf Jaenisch, and Alexander van Oudenaarden. 2017. “Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.” Nature Communications 8 (1): 1096. doi:10.1038/s41467-017-01076-4. http://dx.doi.org/10.1038/s41467-017-01076-4Test.; http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492502Test |
| DOI: | 10.1038/s41467-017-01076-4 |
| الإتاحة: | https://doi.org/10.1038/s41467-017-01076-4Test http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492502Test |
| رقم الانضمام: | edsbas.398616B2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-017-01076-4 |
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