Methylomic and phenotypic analysis of the ModH5 phasevarion of Helicobacter pylori
| العنوان: | Methylomic and phenotypic analysis of the ModH5 phasevarion of Helicobacter pylori |
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| المؤلفون: | Peter M. Power, Rebecca J. Gorrell, Matthew Boitano, Tyson A. Clark, Yogitha N. Srikhanta, Terry Kwok, Elizabeth L. Hartland, Kirill Tsyganov, Jonas Korlach, Michael P. Jennings |
| المصدر: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
| بيانات النشر: | Nature Publishing Group UK, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Phase variation, Regulation of gene expression, Genetics, Multidisciplinary, biology, Helicobacter pylori, lcsh:R, 030106 microbiology, Mutant, lcsh:Medicine, Gene Expression Regulation, Bacterial, Article, Epigenesis, Genetic, 03 medical and health sciences, 030104 developmental biology, Bacterial Proteins, Gene expression, DNA methylation, biology.protein, lcsh:Q, Epigenetics, lcsh:Science, Gene, Flagellin |
| الوصف: | The Helicobacter pylori phase variable gene modH, typified by gene HP1522 in strain 26695, encodes a N6-adenosine type III DNA methyltransferase. Our previous studies identified multiple strain-specific modH variants (modH1 – modH19) and showed that phase variation of modH5 in H. pylori P12 influenced expression of motility-associated genes and outer membrane protein gene hopG. However, the ModH5 DNA recognition motif and the mechanism by which ModH5 controls gene expression were unknown. Here, using comparative single molecule real-time sequencing, we identify the DNA site methylated by ModH5 as 5′-Gm6ACC-3′. This motif is vastly underrepresented in H. pylori genomes, but overrepresented in a number of virulence genes, including motility-associated genes, and outer membrane protein genes. Motility and the number of flagella of H. pylori P12 wild-type were significantly higher than that of isogenic modH5 OFF or ΔmodH5 mutants, indicating that phase variable switching of modH5 expression plays a role in regulating H. pylori motility phenotypes. Using the flagellin A (flaA) gene as a model, we show that ModH5 modulates flaA promoter activity in a GACC methylation-dependent manner. These findings provide novel insights into the role of ModH5 in gene regulation and how it mediates epigenetic regulation of H. pylori motility. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bde49c6d51c1764265c234a41671664fTest http://europepmc.org/articles/PMC5700931Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bde49c6d51c1764265c234a41671664f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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