Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes
| العنوان: | Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes |
|---|---|
| المؤلفون: | Tomasz Budlewski, Piotr Kosielski, Gianluca Padula, Pawel Meissner, Konrad Walczak, Anna Wlodarczyk, Agata Sarniak, Robert Stawski, Dariusz Nowak, Ewelina Perdas |
| المصدر: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| بيانات النشر: | Nature Publishing Group UK, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Mitochondrial DNA, Phagocyte, lcsh:Medicine, Negative association, Plasma cell, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, medicine, DNA metabolism, Author Correction, lcsh:Science, Whole blood, chemistry.chemical_classification, Reactive oxygen species, Multidisciplinary, lcsh:R, 030229 sport sciences, Circulating Cell-Free DNA, Nuclear DNA, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, chemistry, lcsh:Q, human activities, Biological fluorescence, DNA |
| الوصف: | Objective Strenuous exercise increases circulating cell free DNA (cf DNA) and stimulates blood phagocytes to generate more reactive oxygen species (ROS) which may induce DNA strand breaks. We tested whether: (A) elevated cf DNA in response to three repeated bouts of exhaustive exercise has decreased integrity; (B) each bout of exercise increases luminol enhanced whole blood chemiluminescence (LBCL) as a measure of ROS production by polymorphonuclear leukocytes; (C) there is an association between integrity of cf DNA and LBCL. Methods Eleven average-trained men performed three treadmill exercise tests to exhaustion at speed corresponding to 70% of their individual VO2max separated by 72 hours of resting. Pre- and post-exercise concentrations and integrity of cf nuclear and mitochondrial DNA (cf n-DNA, cf mt-DNA) were determined with real-time PCR along with measurement of resting and fMLP-stimulated LBCL. Results Each bout increased concentrations of cf n-DNA by more than 10-times which was accompanied by about 2-times elevated post-exercise rLBCL and fMLP-LBCL (p |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52691f8b60f074ca0eaa7ca46a309a3eTest http://europepmc.org/articles/PMC6828751Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....52691f8b60f074ca0eaa7ca46a309a3e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
|---|