Chondrogenic and BMP-4 primings confer osteogenesis potential to human cord blood mesenchymal stromal cells delivered with biphasic calcium phosphate ceramics
| العنوان: | Chondrogenic and BMP-4 primings confer osteogenesis potential to human cord blood mesenchymal stromal cells delivered with biphasic calcium phosphate ceramics |
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| المؤلفون: | Meadhbh Á. Brennan, Mario Barilani, Francesco Rusconi, Julien de Lima, Luciano Vidal, Cristiana Lavazza, Lorenza Lazzari, Rosaria Giordano, Pierre Layrolle |
| المصدر: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| بيانات النشر: | Nature Publishing Group UK, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Science, Stem-cell differentiation, Stem cells, Bone Morphogenetic Protein 4, Mesenchymal Stem Cell Transplantation, Article, Young Adult, stomatognathic system, Osteogenesis, Humans, Cells, Cultured, Tissue Engineering, Cell Differentiation, Mesenchymal Stem Cells, Fetal Blood, Immunohistochemistry, Stem-cell research, Multipotent stem cells, Bone Substitutes, Medicine, Cytokines, Hydroxyapatites, Biomedical engineering, Chondrogenesis, Biomarkers |
| الوصف: | Bone marrow mesenchymal stem/stromal cells (BMSCs) show great promise for bone repair, however they are isolated by an invasive bone marrow harvest and their regenerative potential decreases with age. Conversely, cord blood can be collected non-invasively after birth and contains MSCs (CBMSCs) that can be stored for future use. However, whether CBMSCs can replace BMSCs targeting bone repair is unknown. This study evaluates the in vitro osteogenic potential of unprimed, osteogenically primed, or chondrogenically primed CBMSCs and BMSCs and their in vivo bone forming capacity following ectopic implantation on biphasic calcium phosphate ceramics in nude mice. In vitro, alkaline phosphatase (intracellular, extracellular, and gene expression), and secretion of osteogenic cytokines (osteoprotegerin and osteocalcin) was significantly higher in BMSCs compared with CBMSCs, while CBMSCs demonstrated superior chondrogenic differentiation and secretion of interleukins IL-6 and IL-8. BMSCs yielded significantly more cell engraftment and ectopic bone formation compared to CBMSCs. However, priming of CBMSCs with either chondrogenic or BMP-4 supplements led to bone formation by CBMSCs. This study is the first direct quantification of the bone forming abilities of BMSCs and CBMSCs in vivo and, while revealing the innate superiority of BMSCs for bone repair, it provides avenues to induce osteogenesis by CBMSCs. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::c265db538ec4cb0fb3175913a1985edeTest http://europepmc.org/articles/PMC7991626Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....c265db538ec4cb0fb3175913a1985ede |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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