Embryonic periventricular endothelial cells demonstrate a unique pro-neurodevelopment and anti-inflammatory gene signature
| العنوان: | Embryonic periventricular endothelial cells demonstrate a unique pro-neurodevelopment and anti-inflammatory gene signature |
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| المؤلفون: | Mauricio A. Contreras, Ravi Vissapragada, Cleide Angolano, Manoj Bhasin, Christiane Ferran, Franciele Cristina Kipper, Justin M. Moore, Ajith J. Thomas |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) |
| بيانات النشر: | Nature Publishing Group UK, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Aging, Science, Neurogenesis, Primary Cell Culture, Biology, Fibroblast growth factor, Article, Cell Line, Transcriptome, Mice, Neural Stem Cells, Interferon, medicine, Animals, Multidisciplinary, Gene Expression Profiling, Wnt signaling pathway, Brain, Endothelial Cells, Gene Expression Regulation, Developmental, Molecular Sequence Annotation, Mouse Embryonic Stem Cells, Complement System Proteins, Gene signature, Embryo, Mammalian, Hypoxia-Inducible Factor 1, alpha Subunit, Embryonic stem cell, Neural stem cell, Coculture Techniques, Cell biology, Mice, Inbred C57BL, Wnt Proteins, HIF1A, Gene Ontology, Medicine, Fibroblast Growth Factor 1, Female, Angiogenesis, Interferons, Carrier Proteins, Stem-cell niche, medicine.drug |
| الوصف: | Brain embryonic periventricular endothelial cells (PVEC) crosstalk with neural progenitor cells (NPC) promoting mutual proliferation, formation of tubular-like structures in the former and maintenance of stemness in the latter. To better characterize this interaction, we conducted a comparative transcriptome analysis of mouse PVEC vs. adult brain endothelial cells (ABEC) in mono-culture or NPC co-culture. We identified > 6000 differentially expressed genes (DEG), regardless of culture condition. PVEC exhibited a 30-fold greater response to NPC than ABEC (411 vs. 13 DEG). Gene Ontology (GO) analysis of DEG that were higher or lower in PVEC vs. ABEC identified “Nervous system development” and “Response to Stress” as the top significantly different biological process, respectively. Enrichment in canonical pathways included HIF1A, FGF/stemness, WNT signaling, interferon signaling and complement. Solute carriers (SLC) and ABC transporters represented an important subset of DEG, underscoring PVEC’s implication in blood–brain barrier formation and maintenance of nutrient-rich/non-toxic environment. Our work characterizes the gene signature of PVEC and their important partnership with NPC, underpinning their unique role in maintaining a healthy neurovascular niche, and in supporting brain development. This information may pave the way for additional studies to explore their therapeutic potential in neuro-degenerative diseases, such as Alzheimer’s and Parkinson’s disease. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55a55240e85ce5845cab7f3db28e9062Test http://europepmc.org/articles/PMC7683543Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....55a55240e85ce5845cab7f3db28e9062 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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