Akt regulates neurite growth by phosphorylation-dependent inhibition of radixin proteasomal degradation
| العنوان: | Akt regulates neurite growth by phosphorylation-dependent inhibition of radixin proteasomal degradation |
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| المؤلفون: | Jee-Yin Ahn, Inwoo Hwang, Kye Won Park, Jeong-Yun Choi, Byeong-Seong Kim, Sung-Woo Cho, Hyo Rim Ko, Eun-Ju Jin |
| المصدر: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| بيانات النشر: | Nature Publishing Group UK, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Proteasome Endopeptidase Complex, Neurite, Moesin, Science, Neurogenesis, Growth Cones, Neuronal Outgrowth, macromolecular substances, Filamentous actin, PC12 Cells, Article, 03 medical and health sciences, Mice, Radixin, Animals, Humans, Phosphorylation, Growth cone, Protein kinase B, Multidisciplinary, 030102 biochemistry & molecular biology, Chemistry, Protein Stability, Membrane Proteins, Actins, Cell biology, Rats, Cytoskeletal Proteins, 030104 developmental biology, HEK293 Cells, Proteolysis, Medicine, Signal transduction, Proto-Oncogene Proteins c-akt, Protein Binding, Signal Transduction |
| الوصف: | Neurite growth is controlled by a complex molecular signaling network that regulates filamentous actin (F-actin) dynamics at the growth cone. The evolutionarily conserved ezrin, radixin, and moesin family of proteins tether F-actin to the cell membrane when phosphorylated at a conserved threonine residue and modulate neurite outgrowth. Here we show that Akt binds to and phosphorylates a threonine 573 residue on radixin. Akt-mediated phosphorylation protects radixin from ubiquitin-dependent proteasomal degradation, thereby enhancing radixin protein stability, which permits proper neurite outgrowth and growth cone formation. Conversely, the inhibition of Akt kinase or disruption of Akt-dependent phosphorylation reduces the binding affinity of radixin to F-actin as well as lowers radixin protein levels, resulting in decreased neurite outgrowth and growth cone formation. Our findings suggest that Akt signaling regulates neurite outgrowth by stabilizing radixin interactions with F-actin, thus facilitating local F-actin dynamics. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::832ca8e43b83f2d44bac9bce8bee9f26Test http://europepmc.org/articles/PMC5803261Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....832ca8e43b83f2d44bac9bce8bee9f26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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