دورية أكاديمية
Small secreted proteins from the necrotrophic conifer pathogen Heterobasidion annosum s. l. (HaSSPs) induce cell death in Nicotiana benthamiana
| العنوان: | Small secreted proteins from the necrotrophic conifer pathogen Heterobasidion annosum s. l. (HaSSPs) induce cell death in Nicotiana benthamiana |
|---|---|
| المؤلفون: | Raffaello, Tommaso, Asiegbu, Fred O. |
| المساهمون: | Department of Forest Sciences, Frederick Asiegbu / Principal Investigator, Viikki Plant Science Centre (ViPS), Forest Ecology and Management |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2017 |
| المجموعة: | Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: | 4112 Forestry, CHITIN-TRIGGERED IMMUNITY, SUBCELLULAR-LOCALIZATION, TRANSCRIPTION FACTOR, EFFECTOR PROTEINS, ARABIDOPSIS, EXPRESSION, DEFENSE, GENOME, RESISTANCE, PREDICTION |
| الوصف: | The basidiomycete Heterobasidion annosum sensu lato (s. l.) is considered to be one of the most destructive conifer pathogens in the temperate forests of the northern hemisphere. H. annosum is characterized by a dual fungal lifestyle. The fungus grows necrotrophically on living plant cells and saprotrophically on dead wood material. In this study, we screened the H. annosum genome for small secreted proteins (HaSSPs) that could potentially be involved in promoting necrotrophic growth during the fungal infection process. The final list included 58 HaSSPs that lacked predictable protein domains. The transient expression of HaSSP encoding genes revealed the ability of 8 HaSSPs to induce cell chlorosis and cell death in Nicotiana benthamiana. In particular, one protein (HaSSP30) could induce a rapid, strong, and consistent cell death within 2 days post-infiltration. HaSSP30 also increased the transcription of host-defence-related genes in N. benthamiana, which suggested a necrotrophic-specific immune response. This is the first line of evidence demonstrating that the H. annosum genome encodes HaSSPs with the capability to induce plant cell death in a non-host plant. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-0-00-407444-3 0-00-407444-0 |
| العلاقة: | This research was supported by grants from the Academy of Finland (project no. 276862). We also thank Dr. Benjamin Petre and Prof. Sophien Kamoun (The Sainsbury Laboratory, Norwich, UK) for assistance with the cloning design and for further verification and validation of HaSSP30. We gratefully acknowledge the Heterobasidion Genome Consortium for access to the published data.; Raffaello , T & Asiegbu , F O 2017 , ' Small secreted proteins from the necrotrophic conifer pathogen Heterobasidion annosum s. l. (HaSSPs) induce cell death in Nicotiana benthamiana ' , Scientific Reports , vol. 7 , 8000 . https://doi.org/10.1038/s41598-017-08010-0Test; ORCID: /0000-0002-4074-0682/work/39204075; ORCID: /0000-0003-0223-7194/work/51559859; 85032995125; a94f93a9-0072-453e-b1ff-918e64b64804; http://hdl.handle.net/10138/223786Test; 000407444000007 |
| الإتاحة: | http://hdl.handle.net/10138/223786Test |
| حقوق: | cc_by ; openAccess ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6BA0C990 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780004074443 0004074440 |
|---|