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Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner

التفاصيل البيبلوغرافية
العنوان: Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner
المؤلفون: Yujian Wang, Jingchang Luo, Xiaolu Guan, Yan Zhao, Li Sun
المصدر: Cell Death Discovery, Vol 10, Iss 1, Pp 1-9 (2024)
بيانات النشر: Nature Publishing Group, 2024.
سنة النشر: 2024
المجموعة: LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
LCC:Cytology
مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671
الوصف: Abstract Bacillus cereus is a clinically significant foodborne pathogen that causes severe gastrointestinal and non-gastrointestinal disease. Cereolysin O (CLO) is a putative virulence factor of B. cereus, and its function remains to be investigated. In this study, we examined the biological activity of CLO from a deep sea B. cereus isolate. CLO was highly toxic to mammalian cells and triggered pyroptosis through NLRP3 inflammasome-mediated caspase 1 and gasdermin D activation. CLO-induced cell death involved ROS accumulation and K+ efflux, and was blocked by serum lipids. CLO bound specifically to cholesterol, and this binding was essential to CLO cytotoxicity. The structural integrity of the three tryptophan residues in the C-terminal undecapeptide was vital for CLO to interact with membrane lipids and cause membrane perforation. Taken together, these results provided new insights into the molecular mechanism of B. cereus CLO-mediated cytotoxicity.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2058-7716
العلاقة: https://doaj.org/toc/2058-7716Test
DOI: 10.1038/s41420-024-01887-7
الوصول الحر: https://doaj.org/article/d9aa71cee06a44f7b259d7bb1b4f7a1aTest
رقم الانضمام: edsdoj.9aa71cee06a44f7b259d7bb1b4f7a1a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20587716
DOI:10.1038/s41420-024-01887-7