Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact
| العنوان: | Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact |
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| المؤلفون: | Julie Meurisse, Ramona Schuster, Chen Bibi, Lodewijk IJlst, Nadav Shai, Nir Cohen, Muriel Mari, Einat Zalckvar, Mafalda Escobar-Henriques, Hans R. Waterham, Adam Hughes, Laetitia Cavellini, Eden Yifrach, Mickael M. Cohen, Maya Schuldiner, Carlo W.T. van Roermund, Ronald J.A. Wanders, Lior Zada, Fulvio Reggiori |
| المساهمون: | Weizmann Institute of Science [Rehovot, Israël], University of Amsterdam [Amsterdam] (UvA), Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Cologne, University Medical Center Groningen [Groningen] (UMCG), University of Utah School of Medicine [Salt Lake City], Laboratory Genetic Metabolic Diseases, AGEM - Inborn errors of metabolism, APH - Methodology, Microbes in Health and Disease (MHD), Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| المصدر: | Nature Communications Nature Communications, Nature Publishing Group, 2018, 9, pp.1761. ⟨10.1038/s41467-018-03957-8⟩ Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018) Nature communications, 9(1):1761. Nature Publishing Group Shai, N, Yifrach, E, van Roermund, C W T, Cohen, N, Bibi, C, IJlst, L, Cavellini, L, Meurisse, J, Schuster, R, Zada, L, Mari, M C, Reggiori, F M, Hughes, A L, Escobar-Henriques, M, Cohen, M M, Waterham, H R, Wanders, R J A, Schuldiner, M & Zalckvar, E 2018, ' Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact ', Nature Communications, vol. 9, no. 1, pp. 1761 . https://doi.org/10.1038/s41467-018-03957-8Test Nature Communications, 9(1):1761. Nature Publishing Group |
| بيانات النشر: | Nature Publishing Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Intracellular Membranes/metabolism, 0301 basic medicine, Cytoplasm, General Physics and Astronomy, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Mitochondrion, GTP Phosphohydrolases, Peroxins, SACCHAROMYCES-CEREVISIAE, Bimolecular fluorescence complementation, PROTEIN-PROTEIN INTERACTIONS, Saccharomyces cerevisiae/metabolism, 0302 clinical medicine, FUSION, Protein Interaction Mapping, lcsh:Science, Multidisciplinary, Chemistry, Tethering, High-throughput screening, Peroxins/metabolism, Peroxisome, Saccharomyces cerevisiae Proteins/metabolism, Cytoplasm/metabolism, 3. Good health, Mitochondria, Systematic mapping, Protein Binding, Mitochondrial Proteins/metabolism, Saccharomyces cerevisiae Proteins, Science, MITOFUSIN, SIGNAL PEPTIDE PREDICTION, Saccharomyces cerevisiae, General Biochemistry, Genetics and Molecular Biology, Article, Protein–protein interaction, Mitochondrial Proteins, 03 medical and health sciences, Mitochondria/metabolism, Peroxisomes, YEAST LIBRARIES, Organelles, Binding Sites, BIMOLECULAR FLUORESCENCE COMPLEMENTATION, Peroxisomes/metabolism, Membrane Proteins, General Chemistry, Intracellular Membranes, COMBINED TRANSMEMBRANE TOPOLOGY, 030104 developmental biology, ER, PLASMA-MEMBRANE, Biophysics, Proximity detection, lcsh:Q, Membrane Proteins/metabolism, GTP Phosphohydrolases/metabolism, 030217 neurology & neurosurgery, Function (biology) |
| الوصف: | The understanding that organelles are not floating in the cytosol, but rather held in an organized yet dynamic interplay through membrane contact sites, is altering the way we grasp cell biological phenomena. However, we still have not identified the entire repertoire of contact sites, their tethering molecules and functions. To systematically characterize contact sites and their tethering molecules here we employ a proximity detection method based on split fluorophores and discover four potential new yeast contact sites. We then focus on a little-studied yet highly disease-relevant contact, the Peroxisome-Mitochondria (PerMit) proximity, and uncover and characterize two tether proteins: Fzo1 and Pex34. We genetically expand the PerMit contact site and demonstrate a physiological function in β-oxidation of fatty acids. Our work showcases how systematic analysis of contact site machinery and functions can deepen our understanding of these structures in health and disease. The internal organization of the cell has been enriched by the discovery that organelles establish membrane contact sites, however the entire repertoire of these contacts is still being explored. Here the authors systematically identify the landscape of cellular contact sites in yeast, discovering four potential novel contact sites and two tether proteins for the peroxisome-mitochondria contact site. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04e072777b98620ce047b021657bd05dTest https://doi.org/10.1038/s41467-018-03957-8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....04e072777b98620ce047b021657bd05d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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