التفاصيل البيبلوغرافية
| العنوان: |
Targeted gene expression profiling for accurate endometrial receptivity testing |
| المؤلفون: |
Meltsov, Alvin, Saare, Merli, Teder, Hindrek, Paluoja, Priit, Arffman, Riikka K., Piltonen, Terhi, Laudanski, Piotr, Wielgos, Miroslaw, Gianaroli, Luca, Koel, Mariann, Peters, Maire, Salumets, Andres, Krjutskov, Kaarel, Palta, Priit |
| المساهمون: |
Institute for Molecular Medicine Finland, Genomics of Neurological and Neuropsychiatric Disorders |
| بيانات النشر: |
Nature Publishing Group |
| سنة النشر: |
2024 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
Embryo-transfer, Frozen, Cycles, Window, Array, 1184 Genetics, developmental biology, physiology |
| الوصف: |
Expressional profiling of the endometrium enables the personalised timing of the window of implantation (WOI). This study presents and evaluates a novel analytical pipeline based on a TAC-seq (Targeted Allele Counting by sequencing) method for endometrial dating. The expressional profiles were clustered, and differential expression analysis was performed on the model development group, using 63 endometrial biopsies spanning over proliferative (PE, n = 18), early-secretory (ESE, n = 18), mid-secretory (MSE, n = 17) and late-secretory (LSE, n = 10) endometrial phases of the natural cycle. A quantitative predictor model was trained on the development group and validated on sequenced samples from healthy women, consisting of 52 paired samples taken from ESE and MSE phases and five LSE phase samples from 31 individuals. Finally, the developed test was applied to 44 MSE phase samples from a study group of patients diagnosed with recurrent implantation failure (RIF). In validation samples (n = 57), we detected displaced WOI in 1.8% of the samples from fertile women. In the RIF study group, we detected a significantly higher proportion of the samples with shifted WOI than in the validation set of samples from fertile women, 15.9% and 1.8% (p = 0.012), respectively. The developed model was evaluated with an average cross-validation accuracy of 98.8% and an accuracy of 98.2% in the validation group. The developed beREADY screening model enables sensitive and dynamic detection of selected transcriptome biomarkers, providing a quantitative and accurate prediction of endometrial receptivity status. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
This research was funded by the Estonian Research Council (grant PRG1076), Horizon 2020 innovation grant (ERIN, grant no. EU952516), the EU-FP7 Marie Curie Industry-Academia Partnerships and Pathways (IAPP, grant SARM, EU324509), the Enterprise Estonia (grant no. EU48695) and by Polish Ministry of Health (grant no. 6/6/4/1/NPZ/2017/1210/1352).; Meltsov , A , Saare , M , Teder , H , Paluoja , P , Arffman , R K , Piltonen , T , Laudanski , P , Wielgos , M , Gianaroli , L , Koel , M , Peters , M , Salumets , A , Krjutskov , K & Palta , P 2023 , ' Targeted gene expression profiling for accurate endometrial receptivity testing ' , Scientific Reports , vol. 13 , no. 1 , 13959 . https://doi.org/10.1038/s41598-023-40991-zTest; ORCID: /0000-0001-9320-7008/work/153479395; 85168754103; bc8306b3-aa36-4111-a28b-8f300fa2ab2b; http://hdl.handle.net/10138/571101Test; 001138684400001 |
| الإتاحة: |
http://hdl.handle.net/10138/571101Test |
| حقوق: |
cc_by ; openAccess ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.467CDDCA |
| قاعدة البيانات: |
BASE |
