دورية أكاديمية
Anti-Hu antibodies activate enteric and sensory neurons.
العنوان: | Anti-Hu antibodies activate enteric and sensory neurons. |
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المؤلفون: | Li, Q., Michel, K., Annahazi, A., Demir, I.E., Ceyhan, G.O., Zeller, F., Komorowski, L., Stöcker, W., Beyak, M.J., Grundy, D., Farrugia, G., De Giorgio, R., Schemann, M. |
بيانات النشر: | Nature Publishing Group |
سنة النشر: | 2016 |
المجموعة: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
الوصف: | IgG of type 1 anti-neuronal nuclear antibody (ANNA-1, anti-Hu) specificity is a serological marker of paraneoplastic neurological autoimmunity (including enteric/autonomic) usually related to small-cell lung carcinoma. We show here that IgG isolated from such sera and also affinity-purified anti-HuD label enteric neurons and cause an immediate spike discharge in enteric and visceral sensory neurons. Both labelling and activation of enteric neurons was prevented by preincubation with the HuD antigen. Activation of enteric neurons was inhibited by the nicotinic receptor antagonists hexamethonium and dihydro-β-erythroidine and reduced by the P2X antagonist pyridoxal phosphate-6-azo (benzene-2,4-disulfonic acid (PPADS) but not by the 5-HT3 antagonist tropisetron or the N-type Ca-channel blocker ω-Conotoxin GVIA. Ca(++) imaging experiments confirmed activation of enteric neurons but not enteric glia. These findings demonstrate a direct excitatory action of ANNA-1, in particular anti-HuD, on visceral sensory and enteric neurons, which involves nicotinic and P2X receptors. The results provide evidence for a novel link between nerve activation and symptom generation in patients with antibody-mediated gut dysfunction. |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | text |
اللغة: | English |
العلاقة: | https://eprints.whiterose.ac.uk/109122/1/anti%20hu%20antibodies.pdfTest; Li, Q., Michel, K., Annahazi, A. et al. (10 more authors) (2016) Anti-Hu antibodies activate enteric and sensory neurons. Scientific Reports, 6. 38216. ISSN 2045-2322 |
DOI: | 10.1038/srep38216 |
الإتاحة: | https://doi.org/10.1038/srep38216Test https://eprints.whiterose.ac.uk/109122Test/ https://eprints.whiterose.ac.uk/109122/1/anti%20hu%20antibodies.pdfTest |
حقوق: | cc_by_4 |
رقم الانضمام: | edsbas.8490B4FF |
قاعدة البيانات: | BASE |
DOI: | 10.1038/srep38216 |
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