CLIC1 and CLIC4 complement CA125 as a diagnostic biomarker panel for all subtypes of epithelial ovarian cancer
| العنوان: | CLIC1 and CLIC4 complement CA125 as a diagnostic biomarker panel for all subtypes of epithelial ovarian cancer |
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| المؤلفون: | Ronny Drapkin, Benjamin G. Bitler, Bipradeb Singha, Mark E. Borowsky, Katherine M. Aird, Qin Liu, Stephanie Jean, Sandra L. Harper, David W. Speicher, Mark G. Cadungog, Aaron R. Goldman, Rugang Zhang |
| المصدر: | Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Stromal cell, endocrine system diseases, lcsh:Medicine, Carcinoma, Ovarian Epithelial, Organ culture, Article, 03 medical and health sciences, 0302 clinical medicine, Chloride Channels, Biomarkers, Tumor, Carcinoma, medicine, Humans, lcsh:Science, Aged, Gene knockdown, Multidisciplinary, Cell growth, business.industry, lcsh:R, Membrane Proteins, Middle Aged, medicine.disease, female genital diseases and pregnancy complications, 3. Good health, Staining, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Tumor progression, CA-125 Antigen, 030220 oncology & carcinogenesis, Cancer research, Biomarker (medicine), Female, lcsh:Q, business |
| الوصف: | New plasma and tissue biomarkers of epithelial ovarian cancer (EOC) could improve early diagnosis and post-diagnosis clinical management. Here we investigated tissue staining and tissue secretion of CLIC1 and CLIC4 across EOC subtypes. CLIC1 and CLIC4 are two promising biomarkers we previously showed were elevated in EOC patient sera. Individually, CLIC1 or CLIC4 stained larger percentages of malignant tumors across all EOC subtypes compared with CA125, particularly early stage and mucinous tumors. CLIC4 also stained benign tumors but staining was limited to nuclei; whereas malignant tumors showed diffuse cellular staining of stromal and tumor cells. Both proteins were shed by all EOC subtypes tumors in short term organ culture at more consistent levels than CA125, supporting their potential as pan-subtype serum and tissue biomarkers. Elevated CLIC4 expression, but not CLIC1 expression, was a negative indicator of patient survival, and CLIC4 knockdown in cultured cells decreased cell proliferation and migration indicating a potential role in tumor progression. These results suggest CLIC1 and CLIC4 are promising serum and tissue biomarkers as well as potential therapeutic targets for all EOC subtypes. This justifies development of high throughput serum/plasma biomarker assays to evaluate utility of a biomarker panel consisting of CLIC1, CLIC4 and CA125. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4308cf7955c573ce8505b872cd0a6c88Test http://link.springer.com/article/10.1038/s41598-018-32885-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4308cf7955c573ce8505b872cd0a6c88 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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