Anosmin-1 activates vascular endothelial growth factor receptor and its related signaling pathway for olfactory bulb angiogenesis
| العنوان: | Anosmin-1 activates vascular endothelial growth factor receptor and its related signaling pathway for olfactory bulb angiogenesis |
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| المؤلفون: | Akio Shimizu, Hisakazu Ogita, Masahiro Komeno, Hironao Nakayama, Nobuhiro Ueno, Misuzu Kurokawa-Seo, Hirotsugu Asano, Shoko Matsushima, Manami Kondo, Naoko Sato |
| المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Vascular Endothelial Growth Factor A, Endocrine reproductive disorders, Angiogenesis, Neovascularization, Physiologic, lcsh:Medicine, Nerve Tissue Proteins, Chick Embryo, Article, Neovascularization, Anosmin-1, Cell Movement, Morphogenesis, medicine, Animals, Humans, lcsh:Science, Cells, Cultured, Protein kinase C, Cell Proliferation, Tube formation, Extracellular Matrix Proteins, Body patterning, Multidisciplinary, biology, Chemistry, lcsh:R, Cell migration, Olfactory Bulb, Olfactory bulb, Cell biology, Receptors, Vascular Endothelial Growth Factor, biology.protein, lcsh:Q, Endothelium, Vascular, medicine.symptom, Signal transduction, Signal Transduction |
| الوصف: | Anosmin-1 is a secreted glycoprotein encoded by the ANOS1 gene, and its loss of function causes Kallmann syndrome (KS), which is characterized by anosmia and hypogonadism due to olfactory bulb (OB) dysfunction. However, the physiological function of anosmin-1 remains to be elucidated. In KS, disordered angiogenesis is observed in OB, resulting in its hypoplasia. In this study, we examined the involvement of anosmin-1 in angiogenic processes. Anosmin-1 was detected on the vessel-like structure in OB of chick embryos, and promoted the outgrowth of vascular sprouts as shown by assays of OB tissue culture. Cell migration, proliferation, and tube formation of endothelial cells were induced by treatment with anosmin-1 as well as vascular endothelial growth factor-A (VEGF-A), and further enhanced by treatment with both of them. We newly identified that anosmin-1 activated VEGF receptor-2 (VEGFR2) by binding directly to it, and its downstream signaling molecules, phospholipase Cγ1 (PLCγ1) and protein kinase C (PKC). These results suggest that anosmin-1 plays a key role in the angiogenesis of developing OB through the VEGFR2–PLCγ1–PKC axis by enhancing the VEGF function. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f17ecc3fa5a32d0444dd91ea2d79b4cTest http://link.springer.com/article/10.1038/s41598-019-57040-3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6f17ecc3fa5a32d0444dd91ea2d79b4c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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