المؤلفون: Yujing Fang, Dan Xie, W. M. Zhong, Jianlong Zhang, C. Z. Liang, Jia-Wei Wei, S. Fan, Z. H. Chen, B. Liao, Y. B. Liao, R. H. Wu, H. W. Zhao, J. Z. Cao, J. Pang, B. Li, Dingmei Zhang, Z. T. Tong, Wen Hui Chen, J. Lu, J. H. Luo

المصدر: British Journal of Cancer

مصطلحات موضوعية: STAT3 Transcription Factor, Cancer Research, Pathology, medicine.medical_specialty, Epithelial-Mesenchymal Transition, Cell, Motility, Mice, Nude, Biology, epithelial–mesenchymal transition, STAT3, Transforming Growth Factor beta1, Mice, In vivo, Cell Movement, Peptide Initiation Factors, TGF-β1, EIF5A2, medicine, Biomarkers, Tumor, Animals, Humans, Neoplasm Invasiveness, Epithelial–mesenchymal transition, Molecular Diagnostics, Cells, Cultured, Retrospective Studies, Gene knockdown, Mice, Inbred BALB C, Bladder cancer, RNA-Binding Proteins, aggressiveness, medicine.disease, Prognosis, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Oncology, Urinary Bladder Neoplasms, biology.protein, Cancer research, Immunohistochemistry, bladder cancer, Female

الوصف: Background: EIF5A2, eukaryotic translation initiation factor 5A2, is associated with several human cancers. In this study, we investigated the role of EIF5A2 in the metastatic potential of localised invasive bladder cancer (BC) and its underlying molecular mechanisms were explored. Methods: The expression pattern of EIF5A2 in localised invasive BC was determined by immunohistochemistry. In addition, the function of EIF5A2 in BC and its underlying mechanisms were elucidated with a series of in vitro and in vivo assays. Results: Overexpression of EIF5A2 was an independent predictor for poor metastasis-free survival of localised invasive BC patients treated with radical cystectomy. Knockdown of EIF5A2 inhibited BC cell migratory and invasive capacities in vitro and metastatic potential in vivo and reversed epithelial–mesenchymal transition (EMT), whereas overexpression of EIF5A2 promoted BC cells motility and invasiveness in vitro and metastatic potential in vivo and induced EMT. In addition, we found that EIF5A2 might activate TGF-β1 expression to induce EMT and drive aggressiveness in BC cells. EIF5A2 stabilized STAT3 and stimulated nuclear localisation of STAT3, which resulted in increasing enrichment of STAT3 onto TGF-β1 promoter to enhance the transcription of TGF-β1. Conclusions: EIF5A2 overexpression predicts tumour metastatic potential in patients with localised invasive BC treated with radical cystectomy. Furthermore, EIF5A2 elevated TGF-β1 expression through STAT3 to induce EMT and promotes aggressiveness in BC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68a5cb3d57cb6ff82665a962edbe4a14Test
http://europepmc.org/articles/PMC3974079Test

المؤلفون: Wei Xin, Shuming Zhu, Zhicheng Du, Dingmei Zhang, Yuantao Hao, Yu Deng, Pi Guo, Fangfang Zeng, Yun Huang

المصدر: Scientific Reports

مصطلحات موضوعية: Adult, Male, HBsAg, medicine.medical_specialty, China, Hepatitis B virus, Cross-sectional study, Psychological intervention, medicine.disease_cause, Article, 03 medical and health sciences, 0302 clinical medicine, Epidemiology, medicine, Prevalence, Humans, 030212 general & internal medicine, Child, Multidisciplinary, Hepatitis B Surface Antigens, business.industry, Vaccination, Age Factors, Infant, Newborn, Infant, Hepatitis B, Middle Aged, medicine.disease, Cross-Sectional Studies, Child, Preschool, Immunology, 030211 gastroenterology & hepatology, Cluster sampling, Female, business, Demography

الوصف: Limited information is available about the current epidemic status of hepatitis B virus (HBV) in Guangdong province in South China, where hepatitis B is endemic. We sought to provide an up-to-date assessment of hepatitis B prevalence in a large population through a community-based study. A total of 169,211 local residents were recruited using the stratified cluster random sampling method from 2014 to 2015, and each participant’s information was collected using an interviewer-administered questionnaire. Accordingly, the prevalence of hepatitis B surface antigen (HBsAg) in the total population was 8.76%. HBsAg prevalence was lowest (0.29%) among children aged 0–12 years and highest (12.71%) among those aged 23–59 years. Moreover, the prevalence (8.82%) in males approximately equalled that (8.65%) in females (P > 0.05). Overall, vaccination was effective in preventing HBV infection, regardless of age. Among adults aged 23–59 years, male sex tended to keep the HBsAg persistence. However, reduced persistence for participants with occasional physical exercise and drinking was observed. For participants older than 59 years, a history of prior surgery placed people at high risk for infection. Although Guangdong has successfully decreased the HBsAg prevalence among children, it is urgent to expand vaccination to adults, and employ interventions to reduce the infection risk.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::628182d97f8f91ca177aa39c1e9f9866Test