Selective deletion of the soluble Colony-Stimulating Factor 1 isoform in vivo prevents estrogen-deficiency bone loss in mice
| العنوان: | Selective deletion of the soluble Colony-Stimulating Factor 1 isoform in vivo prevents estrogen-deficiency bone loss in mice |
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| المؤلفون: | Gang-Qing Yao, Nancy Troiano, Christine A. Simpson, Karl L. Insogna |
| المصدر: | Bone Research |
| بيانات النشر: | Nature Publishing Group, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Bone mineral, Macrophage colony-stimulating factor, Gene isoform, medicine.medical_specialty, Histology, Physiology, medicine.drug_class, Chemistry, Endocrinology, Diabetes and Metabolism, Wild type, Bone tissue, Article, Reverse transcription polymerase chain reaction, 03 medical and health sciences, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Estrogen, In vivo, Internal medicine, medicine, Cancer research, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Neutralizing CSF1 in vivo completely prevents ovariectomy (OVX)-induced bone loss in mice. There are two isoforms of CSF1, soluble (sCSF1), and membrane-bound (mCSF1), but their individual biological functions are unclear. It had been previously reported that mCSF1 knockout (K/O) and wild type (Wt) female mice experience the same degree of bone loss following OVX. In Wt mice the expression of sCSF1 was elevated fourfold in skeletal tissue following OVX while expression of mCSF1 was unchanged. To examine the role of sCSF1 in OVX-induced bone loss, mice were engineered in which sCSF1 was not expressed but expression of mCSF1 was unaffected (sCSF1 K/O). Isoform-specific reverse transcription PCR confirmed the absence of transcripts for sCSF1 in bone tissue isolated from these animals and no circulating CSF1 was detected by ELISA. Surprisingly, there were no significant differences in bone mineral density (BMD) between sCSF1 K/O mice and Wt controls as assessed by dual-energy X-ray absorptiometry and micro-CT. However, one month after OVX, femoral, spinal and total BMD had declined by 11.2%, 8.9%, and 8.7% respectively in OVX-Wt animals as compared to Sham-OVX. In contrast OVX sCSF1 K/O mice showed changes of +0.1%, −2.4%, and +2.3% at the same 3 sites compared to Sham-OVX sCSF1 K/O mice. These data indicate important non-redundant functions for the two isoforms of CSF1 and suggest that sCSF1, but not mCSF1, plays a key role in estrogen-deficiency bone loss. |
| اللغة: | English |
| تدمد: | 2095-6231 2095-4700 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a8012fe0277290eb97fcd46989791e0Test http://europepmc.org/articles/PMC5684603Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4a8012fe0277290eb97fcd46989791e0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20956231 20954700 |
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