التفاصيل البيبلوغرافية
| العنوان: |
YAP regulates cell mechanics by controlling focal adhesion assembly |
| المؤلفون: |
Nardone, Giorgia, La Cruz, Jorge Oliver-De, Vrbsky, Jan, Martini, Cecilia, Pribyl, Jan, Skladal, Petr, Pesl, Martin, Caluori, Guido, Pagliari, Stefania, Martino, Fabiana, Maceckova, Zuzana, Hajduch, Marian, Sanz-Garcia, Andres, Pugno, Nicola Maria, Stokin, Gorazd Bernard, Forte, Giancarlo |
| المساهمون: |
Faculty of Pharmacy, University of Helsinki, Division of Pharmaceutical Biosciences |
| بيانات النشر: |
Nature Publishing Group |
| سنة النشر: |
2017 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
SELF-RENEWAL, HIPPO, MIGRATION, PATHWAY, RAC, YAP/TAZ, BINDING, PROTEIN, RHO, MECHANOTRANSDUCTION, 317 Pharmacy |
| الوصف: |
Hippo effectors YAP/TAZ act as on-off mechanosensing switches by sensing modifications in extracellular matrix (ECM) composition and mechanics. The regulation of their activity has been described by a hierarchical model in which elements of Hippo pathway are under the control of focal adhesions (FAs). Here we unveil the molecular mechanism by which cell spreading and RhoA GTPase activity control FA formation through YAP to stabilize the anchorage of the actin cytoskeleton to the cell membrane. This mechanism requires YAP co-transcriptional function and involves the activation of genes encoding for integrins and FA docking proteins. Tuning YAP transcriptional activity leads to the modification of cell mechanics, force development and adhesion strength, and determines cell shape, migration and differentiation. These results provide new insights into the mechanism of YAP mechanosensing activity and qualify this Hippo effector as the key determinant of cell mechanics in response to ECM cues. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
The work was supported by the projects no. LQ1605, LQ1601 and LO1304 from the National Program of Sustainability II or I respectively (MEYS CR), by the project FNUSA-ICRC number CZ.1.05/1.1.00/02.0123 (OP VaVpI) and by CEITEC-Central European Institute of Technology (CZ.1.05/1.1.00/02.0068) from the European Regional Development Fund. Moreover, the work was supported by the European Social Fund and European Regional Development Fund-Project MAGNET (Number CZ.02.1.01/0.0/0.0/15_003/0000492). We are grateful to Katerina Melajova for helping with cell culture, transfection and western blotting, Carmen Escobedo-Lucea for critical reading, Federico Tidu for scanning electron microscopy analysis and Enrico Cenni for helping with image analysis. N.M.P. is supported by the European Research Council PoC 2015 'Silkene' No. 693670, by the European Commission H2020 under the Graphene Flagship Core 1 No. 696656 (WP14 'Polymer Nanocomposites') and under the FET Proactive 'Neurofibres' No. 732344.; Nardone , G , La Cruz , J O-D , Vrbsky , J , Martini , C , Pribyl , J , Skladal , P , Pesl , M , Caluori , G , Pagliari , S , Martino , F , Maceckova , Z , Hajduch , M , Sanz-Garcia , A , Pugno , N M , Stokin , G B & Forte , G 2017 , ' YAP regulates cell mechanics by controlling focal adhesion assembly ' , Nature Communications , vol. 8 , 15321 . https://doi.org/10.1038/ncomms15321Test; ORCID: /0000-0003-0413-4965/work/33744932; 85019410130; bcfbf421-83ef-4bb1-aaec-cc13ec36587a; http://hdl.handle.net/10138/188600Test; 000401279200001 |
| الإتاحة: |
http://hdl.handle.net/10138/188600Test |
| حقوق: |
cc_by ; openAccess ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.50FD6194 |
| قاعدة البيانات: |
BASE |
