Epidermal SIRT1 regulates inflammation, cell migration, and wound healing
| العنوان: | Epidermal SIRT1 regulates inflammation, cell migration, and wound healing |
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| المؤلفون: | Xiaoyang Wu, Yu-Ying He, Ashley Sample, Han Liu, Lei Qiang |
| المصدر: | Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Keratinocytes, 0301 basic medicine, endocrine system diseases, Inflammasomes, Neutrophils, Angiogenesis, lcsh:Medicine, Mice, 0302 clinical medicine, Sirtuin 1, Cell Movement, Transforming Growth Factor beta, Mast Cells, lcsh:Science, Multidisciplinary, biology, integumentary system, Chemistry, Granulation tissue, Cell migration, 3. Good health, Cell biology, medicine.anatomical_structure, Gene Knockdown Techniques, 030220 oncology & carcinogenesis, Sirtuin, Cytokines, medicine.symptom, biological phenomena, cell phenomena, and immunity, hormones, hormone substitutes, and hormone antagonists, Signal Transduction, Epithelial-Mesenchymal Transition, Neovascularization, Physiologic, Inflammation, Article, 03 medical and health sciences, medicine, Animals, Humans, Wound Healing, Epidermis (botany), Macrophages, Regeneration (biology), lcsh:R, Fibroblasts, Oxidative Stress, enzymes and coenzymes (carbohydrates), HEK293 Cells, 030104 developmental biology, Epidermal Cells, Gene Expression Regulation, biology.protein, lcsh:Q, Epidermis, Wound healing |
| الوصف: | Sirtuins (SIRT1-7) are NAD-dependent proteins with the enzymatic activity of deacetylases and ADP ribosyltransferases. SIRT1 is the proto member of the proteins in the mammalian sirtuin family and plays multiple roles in aging and disease. Using mice with epidermis-specific SIRT1 deletion, we show that SIRT1 is required for efficient wound healing. SIRT1 deficiency in the epidermis inhibited the regeneration of both the epidermis and the dermal stroma. SIRT1 loss altered the production of many cytokines, inhibited the recruitment of macrophages, neutrophils, and mast cells, the recruitment and activation of fibroblasts, and angiogenesis in the granulation tissue. In keratinocytes, SIRT1 knockdown inhibited EMT, cell migration, and TGF-β signaling. For the first time, using skin-specific mouse model, we demonstrate that epidermal SIRT1 plays a crucial role in wound repair. These findings are novel in understanding how wound healing is regulated. Our findings provide in vivo and in vitro evidence that SIRT1 in the epidermis regulates cell migration, redox response, inflammation, epidermis re-epithelialization, granulation formation, and proper wound healing in mice. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c9ef626b8b4a42025a5cf5e9fc5f3e2Test http://link.springer.com/article/10.1038/s41598-017-14371-3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9c9ef626b8b4a42025a5cf5e9fc5f3e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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