دورية أكاديمية
ESX1 gene as a potential candidate responsible for male infertility in nonobstructive azoospermia
| العنوان: | ESX1 gene as a potential candidate responsible for male infertility in nonobstructive azoospermia |
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| المؤلفون: | Agnieszka Malcher, Zuzanna Graczyk, Hermann Bauer, Tomasz Stokowy, Andrea Berman, Mikołaj Smolibowski, Dominika Blaszczyk, Piotr Jedrzejczak, Alexander N. Yatsenko, Maciej Kurpisz |
| المصدر: | Scientific Reports, Vol 13, Iss 1, Pp 1-14 (2023) |
| بيانات النشر: | Nature Portfolio, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract Infertility is a problem that affects approximately 15% of couples, and male infertility is responsible for 40–50% of these cases. The cause of male infertility is still poorly diagnosed and treated. One of the prominent causes of male infertility is disturbed spermatogenesis, which can lead to nonobstructive azoospermia (NOA). Whole-genome sequencing (WGS) allows us to identify novel rare variants in potentially NOA-associated genes, among others, in the ESX1 gene. The aim of this study was to activate the ESX1 gene using CRISPRa technology in human germ cells (testicular seminoma cells—TCam-2). Successful activation of the ESX1 gene in TCam-2 cells using the CRISPRa system was achieved, and the expression level of the ESX1 gene was significantly higher in modified TCam-2 cells than in WT cells or the negative control with nontargeted gRNA (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| العلاقة: | https://doaj.org/toc/2045-2322Test |
| DOI: | 10.1038/s41598-023-43854-9 |
| الوصول الحر: | https://doaj.org/article/3d4d30706a7146169ea59dfc53471e2bTest |
| رقم الانضمام: | edsdoj.3d4d30706a7146169ea59dfc53471e2b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-023-43854-9 |