Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent
| العنوان: | Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent |
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| المؤلفون: | Haitham A Bukhary, Mohamed Eltaib Elmobark, Sivakumar Sivagurunathan Moni, Mohammed Ghazwani, Nabil A. Alhakamy, M. Intakhab Alam, Saad S. Alqahtani, Saeed Alshahrani, Muhammad Hadi Sultan, Mohammed Ali Bakkari, Abdulkarim M. Meraya, Osama Madkhali |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Klebsiella pneumoniae, Polymers, Drug Compounding, Science, Proteus vulgaris, 02 engineering and technology, Bacillus subtilis, Microbial Sensitivity Tests, medicine.disease_cause, Gram-Positive Bacteria, Article, Injections, 03 medical and health sciences, Gram-Negative Bacteria, Zeta potential, medicine, Potency, Colloids, Particle Size, Escherichia coli, Antibacterial agent, Multidisciplinary, biology, Chemistry, Dextran Sulfate, 021001 nanoscience & nanotechnology, biology.organism_classification, Anti-Bacterial Agents, 030104 developmental biology, Nanomedicine, Freeze Drying, Staphylococcus aureus, Drug delivery, Nanoparticles, Medicine, 0210 nano-technology, Nuclear chemistry |
| الوصف: | The purpose of this study was to develop a novel nano antibacterial formulation of dextran sulfate sodium polymer. The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial properties, with zeta potential of − 35.2 mV, particle size of 69.3 z d nm, polydispersity index of 0.6, and percentage polydispersity of 77.8. The DSS nanoparticles were stable up to 102 °C. Differential scanning calorimetry revealed an endothermic peak at 165.77 °C in 12.46 min, while XRD analysis at 2θ depicted various peaks at 21.56°, 33.37°, 38.73°, 47.17°, 52.96°, and 58.42°, indicating discrete nanoparticle formation. Antibacterial studies showed that the DSS nanoparticles were effective against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations of DSS nanoparticles for Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae) and Proteus vulgaris (P. vulgaris) were 150, 200, 250, 150, 200, 250, 250 µg/mL, respectively. The antibacterial effects of DSS nanoparticles were in the order E. coli (26 ± 1.2 mm) at 150 µg/mL > S. pyogenes (24.6 ± 0.8 mm) at 250 µg/mL > B. subtilis (23.5 ± 2 mm) at 150 µg/mL > K. pneumoniae (22 ± 2 mm) at 250 µg/mL > P. aeruginosa (21.8 ± 1 mm) at 200 µg/mL > S. aureus (20.8 ± 1 mm) at 200 µg/mL > P. vulgaris (20.5 ± 0.9 mm) at 250 µg/mL. These results demonstrate the antibacterial potency of DSS injectable nanoparticles. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ee5621b62aa5501eed474604e4536bdTest https://doaj.org/article/25aa46f901ae4138b7db51e9670d877bTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0ee5621b62aa5501eed474604e4536bd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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