دورية أكاديمية
Calcium binding at the C-terminus of α-synuclein modulates synaptic vesicle interaction
| العنوان: | Calcium binding at the C-terminus of α-synuclein modulates synaptic vesicle interaction |
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| المؤلفون: | Kaminski, GS, Lautenschlager, Janin, Stephens, Amberley, Fusco, Giuliana, Stroehl, Florian, Curry, Nathan, Zacharopoulou, Maria, Michel, Claire, Laine, Romain, Nespovitaya, Nadezhda, Fantham, Marcus, Pinotsi, Dorothea, Zago, Wagner, Fraser, Paul, Tandon, Anurag, St George-Hyslop, Peter, Rees, Eric, Phillips, Jonathan, De Simone, Alfonso, Kaminski, Clemens |
| بيانات النشر: | Springer Nature Nature Communications |
| سنة النشر: | 2018 |
| المجموعة: | Apollo - University of Cambridge Repository |
| الوصف: | Alpha-synuclein is known to bind to small unilamellar vesicles (SUVs) via its N-terminus which forms an amphipathic alpha-helix upon membrane interaction. Here we show that calcium binds to the C-terminus of alpha-synuclein, therewith increasing its lipid binding capacity. Using CEST-NMR we reveal that alpha-synuclein interacts with isolated synaptic vesicles with two regions, the N-terminus, already known from studies on SUVs, and additionally via its C-terminus, which is regulated by the binding of calcium. Indeed, dSTORM on synaptosomes shows that calcium mediates the localization of alpha-synuclein at the presynaptic terminal, and an imbalance in calcium or alpha-synuclein can cause synaptic vesicle clustering, as seen ex vivo and in vitro. This study provides a new view on the binding of alpha-synuclein to synaptic vesicles, which might also affect our understanding of synucleinopathies. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/274029Test |
| DOI: | 10.17863/CAM.21108 |
| الإتاحة: | https://doi.org/10.17863/CAM.21108Test https://www.repository.cam.ac.uk/handle/1810/274029Test |
| حقوق: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.66CDFBAD |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.21108 |
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