دورية أكاديمية
Phenotypes on demand via switchable target protein degradation in multicellular organisms
| العنوان: | Phenotypes on demand via switchable target protein degradation in multicellular organisms |
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| المؤلفون: | Faden, Frederik, Ramezani, Thomas, Mielke, Stefan, Almudi, Isabel, Nairz, Knud, Froehlich, Marceli S., Höckendorff, Jörg, Brandt, Wolfgang, Hoehenwarter, Wolfgang, Dohmen, R.F., Schnittger, Arp, Dissmeyer, Nico |
| المصدر: | Nature Communications, 7 |
| بيانات النشر: | Nature |
| سنة النشر: | 2016 |
| المجموعة: | ETH Zürich Research Collection |
| الوصف: | Phenotypes on-demand generated by controlling activation and accumulation of proteins of interest are invaluable tools to analyse and engineer biological processes. While temperature-sensitive alleles are frequently used as conditional mutants in microorganisms, they are usually difficult to identify in multicellular species. Here we present a versatile and transferable, genetically stable system based on a low-temperature-controlled N-terminal degradation signal (lt-degron) that allows reversible and switch-like tuning of protein levels under physiological conditions in vivo. Thereby, developmental effects can be triggered and phenotypes on demand generated. The lt-degron was established to produce conditional and cell-type-specific phenotypes and is generally applicable in a wide range of organisms, from eukaryotic microorganisms to plants and poikilothermic animals. We have successfully applied this system to control the abundance and function of transcription factors and different enzymes by tunable protein accumulation. ; ISSN:2041-1723 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/000380838900001; http://hdl.handle.net/20.500.11850/119582Test |
| DOI: | 10.3929/ethz-b-000119582 |
| الإتاحة: | https://doi.org/20.500.11850/119582Test https://doi.org/10.3929/ethz-b-000119582Test https://doi.org/10.1038/ncomms12202Test https://hdl.handle.net/20.500.11850/119582Test |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0Test/ ; Creative Commons Attribution 4.0 International |
| رقم الانضمام: | edsbas.30034B20 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000119582 |
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