دورية أكاديمية
Photosynthetic assimilation of CO2 regulates TOR activity
| العنوان: | Photosynthetic assimilation of CO2 regulates TOR activity |
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| المؤلفون: | Mallén-Ponce, Manuel J., Pérez-Pérez, María Esther, Crespo, José L. |
| المساهمون: | Ministerio de Ciencia y Tecnología (España) |
| بيانات النشر: | National Academy of Sciences (U.S.) |
| سنة النشر: | 2022 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | TOR kinase, CO2, Amino acids, Chlamydomonas |
| الوصف: | The target of rapamycin (TOR) kinase is a master regulator that integrates nutrient signals to promote cell growth in all eukaryotes. It is well established that amino acids and glucose are major regulators of TOR signaling in yeast and metazoan, but whether and how TOR responds to carbon availability in photosynthetic organisms is less understood. In this study, we showed that photosynthetic assimilation of CO2 by the Calvin–Benson–Bassham (CBB) cycle regulates TOR activity in the model single-celled microalga Chlamydomonas reinhardtii. Stimulation of CO2 fixation boosted TOR activity, whereas inhibition of the CBB cycle and photosynthesis down-regulated TOR. We uncovered a tight link between TOR activity and the endogenous level of a set of amino acids including Ala, Glu, Gln, Leu, and Val through the modulation of CO2 fixation and the use of amino acid synthesis inhibitors. Moreover, the finding that the Chlamydomonas starch-deficient mutant sta6 displayed disproportionate TOR activity and high levels of most amino acids, particularly Gln, further connected carbon assimilation and amino acids to TOR signaling. Thus, our results showed that CO2 fixation regulates TOR signaling, likely through the synthesis of key amino acids. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/MCIT/PGC2018-099048-B-I00; Publisher's version; https://doi.org/10.1073/pnas.2115261119Test; Sí; Proceedings of the National Academy of Sciences of the USA, 119(2): e2115261119 (2022); http://hdl.handle.net/10261/258556Test; http://dx.doi.org/10.13039/501100006280Test |
| DOI: | 10.1073/pnas.2115261119 |
| DOI: | 10.13039/501100006280 |
| الإتاحة: | https://doi.org/10.1073/pnas.2115261119Test https://doi.org/10.13039/501100006280Test http://hdl.handle.net/10261/258556Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.13B29D1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1073/pnas.2115261119 |
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