دورية أكاديمية
MicroRNA-dependent metamorphosis in hemimetabolan insects
| العنوان: | MicroRNA-dependent metamorphosis in hemimetabolan insects |
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| المؤلفون: | Gomez-Orte, Eva, Bellés, Xavier |
| بيانات النشر: | National Academy of Sciences (U.S.) |
| سنة النشر: | 2009 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | MicroRNA, Hemimetabolan insects, Metamorphosis |
| الوصف: | How does a juvenile insect transform into an adult? This question, which sums up the wonder of insect metamorphosis, has fascinated mankind since ancient times. Modern physiology has established the endocrine basis regulating these transformations, which mainly depend on two hormone types: ecdysteroids, which promote molts, and juvenile hormones, which repress the transformation into the adult stage. The interplay of these two hormones regulates the genes involved in juvenile and adult programs and the shift from one to the other. microRNAs (miRNAs) are small noncoding RNAs, which participate in many biological processes, and we wondered whether they might be also involved in insect metamorphosis. In insects, Dicer-1 ribonuclease transforms miRNA precursors into mature miRNAs. Thus, using systemic RNA interference (RNAi) to silence the expression of Dicer-1 in the hemimetabolan insect Blattella germanica, we depleted miRNA contents in the last instar nymph. This practically inhibited metamorphosis after the next molt, as the resulting specimens showed nymphoid features and were able to molt again. The experiments show that miRNAs play a key role in hemimetabolan metamorphosis, perhaps regulating genes that are juvenile hormone targets. ; Financial support from the Ministry of Science and Innovation, Spain (project CGL2008–03517/BOS, granted to X.B.) and the Generalitat de Catalunya (2005 SGR 00053), is gratefully acknowledged. E.G.-O. is the recipient of a postdoctoral Consejo Superior de Investigaciones Científicas (I3P program) contract. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 579917 bytes; image/jpeg |
| اللغة: | English |
| تدمد: | 1091-6490 |
| العلاقة: | http://dx.doi.org/10.1073/pnas.0907391106Test; Procedings of the National Academy of Sciences of the United States of America 106: 21678-21682 (2009); http://hdl.handle.net/10261/20063Test |
| DOI: | 10.1073/pnas.0907391106 |
| الإتاحة: | https://doi.org/10.1073/pnas.0907391106Test http://hdl.handle.net/10261/20063Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.14B1F02D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10916490 |
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| DOI: | 10.1073/pnas.0907391106 |