دورية أكاديمية
The human pathobiont Malassezia furfur secreted protease Mfsap1 regulates cell dispersal and exacerbates skin inflammation
| العنوان: | The human pathobiont Malassezia furfur secreted protease Mfsap1 regulates cell dispersal and exacerbates skin inflammation |
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| المؤلفون: | Goh, Joleen P Z, Ruchti, Fiorella, Poh, Si En, Koh, Winston L C, Tan, Kiat Yi, Lim, Yan Ting, Thng, Steven T G, Sobota, Radoslaw M, Hoon, Shawn S, Liu, Chenxi, O’Donoghue, Anthony J, LeibundGut-Landmann, Salomé, Oon, Hazel H, Li, Hao, Dawson, Thomas L |
| المصدر: | Goh, Joleen P Z; Ruchti, Fiorella; Poh, Si En; Koh, Winston L C; Tan, Kiat Yi; Lim, Yan Ting; Thng, Steven T G; Sobota, Radoslaw M; Hoon, Shawn S; Liu, Chenxi; O’Donoghue, Anthony J; LeibundGut-Landmann, Salomé; Oon, Hazel H; Li, Hao; Dawson, Thomas L (2022). The human pathobiont Malassezia furfur secreted protease Mfsap1 regulates cell dispersal and exacerbates skin inflammation. Proceedings of the National Academy of Sciences of the United States of America, 119(49):e2212533119. |
| بيانات النشر: | National Academy of Sciences |
| سنة النشر: | 2022 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Institute of Virology, 570 Life sciences, biology, Multidisciplinary |
| الوصف: | Malassezia form the dominant eukaryotic microbial community on the human skin. The Malassezia genus possesses a repertoire of secretory hydrolytic enzymes involved in protein and lipid metabolism which alter the external cutaneous environment. The exact role of most Malassezia secreted enzymes, including those in interaction with the epithelial surface, is not well characterized. In this study, we compared the expression level of secreted proteases, lipases, phospholipases, and sphingomyelinases of Malassezia globosa in healthy subjects and seborrheic dermatitis or atopic dermatitis patients. We observed upregulated gene expression of the previously characterized secretory aspartyl protease MGSAP1 in both diseased groups, in lesional and non-lesional skin sites, as compared to healthy subjects. To explore the functional roles of MGSAP1 in skin disease, we generated a knockout mutant of the homologous protease MFSAP1 in the genetically tractable Malassezia furfur. We observed the loss of MFSAP1 resulted in dramatic changes in the cell adhesion and dispersal in both culture and a human 3D reconstituted epidermis model. In a murine model of Malassezia colonization, we further demonstrated Mfsap1 contributes to inflammation as observed by reduced edema and inflammatory cell infiltration with the knockout mutant versus wildtype. Taken together, we show that this dominant secretory Malassezia aspartyl protease has an important role in enabling a planktonic cellular state that can potentially aid in colonization and additionally as a virulence factor in barrier-compromised skin, further highlighting the importance of considering the contextual relevance when evaluating the functions of secreted microbial enzymes. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0027-8424 |
| العلاقة: | https://www.zora.uzh.ch/id/eprint/226346/1/pnas.2212533119.pdfTest; info:pmid/36442106; urn:issn:0027-8424 |
| DOI: | 10.5167/uzh-226346 |
| DOI: | 10.1073/pnas.2212533119 |
| الإتاحة: | https://doi.org/10.5167/uzh-22634610.1073/pnas.2212533119Test https://www.zora.uzh.ch/id/eprint/226346Test/ https://www.zora.uzh.ch/id/eprint/226346/1/pnas.2212533119.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.ACAF146E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00278424 |
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| DOI: | 10.5167/uzh-226346 |