التفاصيل البيبلوغرافية
| العنوان: |
Ets-1 facilitates nuclear entry of NFAT proteins and their recruitment to the IL-2 promoter. |
| المؤلفون: |
Hsiao-Wei Tsao, Tzong-Shyuan Tai, Tseng, William, Hui-Hsin Chang, Grenningloh, Roland, Shi-Chuen Miaw, l-Cheng Ho |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 9/24/2013, Vol. 110 Issue 39, p15776-15781, 6p |
| مصطلحات موضوعية: |
NUCLEAR factor of activated T-cells, TRANSCRIPTION factors, IMMUNE response, NUCLEIC acids, PROTEINS, RNA, BIOCHEMISTRY |
| مستخلص: |
E26 transformation-specific sequence 1 (Ets-1), the prototype of the ETS family of transcription factors, is critical for the expression of IL-2 by murine Th cells; however, its mechanism of action is still unclear. Here we show that Ets-1 is also essential for optimal production of IL-2 by primary human Th cells. Although Ets-1 negatively regulates the expression of Blimpl, a known suppressor of IL-2 expression, ablation of B lymphocyte-induced maturation protein 1 (Blimpl) does not rescue the expression of IL-2 by Ets-1-deficient Th cells. Instead, Ets-1 physically and functionally interacts with the nuclear factor of activated T-cells (NFAT) and is required for the recruitment of NFAT to the IL-2 promoter. In addition, Ets-1 is located in both the nucleus and cytoplasm of resting Th cells. Nuclear Ets-1 quickly exits the nucleus in response to calcium-dependent signals and competes with NFAT proteins for binding to protein components of noncoding RNA repressor of NFAT complex (NRON), which serves as a cytoplasmic trap for phosphorylated NFAT proteins. This nuclear exit of Ets-1 precedes rapid nuclear entry of NFAT and Ets-1 deficiency results in impaired nuclear entry, but not dephosphorylation, of NFAT proteins. Thus, Ets-1 promotes the expression of IL-2 by modulating the activity of NFAT. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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