دورية أكاديمية
TAp73 regulates the spindle assembly checkpoint by modulating BubR1 activity
| العنوان: | TAp73 regulates the spindle assembly checkpoint by modulating BubR1 activity |
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| المؤلفون: | Tomasini R., Tsuchihara K., Tsuda C., Lau S. K., Wilhelm M., Ruffini A., Tsao M. S., Iovanna J. L., Jurisicova A., Maka T. W., MELINO, GENNARO |
| المساهمون: | Tomasini, R, Tsuchihara, K, Tsuda, C, Lau, Sk, Wilhelm, M, Ruffini, A, Tsao, M, Iovanna, Jl, Jurisicova, A, Melino, G, Maka, Tw |
| بيانات النشر: | National Academy of Sciences WASHINGTON |
| سنة النشر: | 2009 |
| المجموعة: | Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
| مصطلحات موضوعية: | Bub1, Meiosi, Mitotic arrest, p73, Spindle checkpoint, Settore BIO/11 - BIOLOGIA MOLECOLARE |
| الوصف: | The role of various p73 isoforms in tumorigenesis has been controversial. However, as we have recently shown, the generation of TAp73-deficient (TAp73(-/-)) mice reveals that TAp73 isoforms exert tumor-suppressive functions, indicating an emerging role for Trp-73 in the maintenance of genomic stability. Unlike mice lacking all p73 isoforms, TAp73(-/-) mice show a high incidence of spontaneous tumors. Moreover, TAp73(-/-) mice are infertile and produce oocytes exhibiting spindle abnormalities. These data suggest a link between TAp73 activities and the common molecular machinery underlying meiosis and mitosis. Previous studies have indicated that the spindle assembly checkpoint (SAC) complex, whose activation leads to mitotic arrest, also regulates meiosis. In this study, we demonstrate in murine and human cells that TAp73 is able to interact directly with several partners of the SAC complex (Bub1, Bub3, and BubR1). We also show that TAp73 is involved in SAC protein localization and activities. Moreover, we show that decreased TAp73 expression correlates with increases of SAC protein expression in patients with lung cancer. Our results establish TAp73 as a regulator of SAC responses and indicate that TAp73 loss can lead to mitotic arrest defects. Our data suggest that SAC impairment in the absence of functional TAp73 could explain the genomic instability and increased aneuploidy observed in TAp73-deficient cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/19139399; info:eu-repo/semantics/altIdentifier/wos/WOS:000262809700024; volume:106; issue:3; firstpage:797; lastpage:802; journal:PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; http://hdl.handle.net/2108/47260Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-58849134327 |
| DOI: | 10.1073/pnas.0812096106 |
| الإتاحة: | https://doi.org/10.1073/pnas.0812096106Test http://hdl.handle.net/2108/47260Test |
| رقم الانضمام: | edsbas.B47D461B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1073/pnas.0812096106 |
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