دورية أكاديمية
Dynamical control enables the formation of demixed biomolecular condensates.
| العنوان: | Dynamical control enables the formation of demixed biomolecular condensates. |
|---|---|
| المؤلفون: | Lin, Andrew Z, Ruff, Kiersten M, Dar, Furqan, Jalihal, Ameya, King, Matthew R, Lalmansingh, Jared M, Posey, Ammon E, Erkamp, Nadia A, Seim, Ian, Gladfelter, Amy S, Pappu, Rohit V |
| بيانات النشر: | Springer Science and Business Media LLC //dx.doi.org/10.1038/s41467-023-43489-4 Nat Commun |
| سنة النشر: | 2023 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Biomolecular Condensates, RNA, Thermodynamics |
| الوصف: | Acknowledgements: This work was supported by grants from the Air Force Office of Scientific Research (grant FA9550-20-1-0241 to A.S.G. and R.V.P.), the St. Jude Research Collaborative on the Biology and Biophysics of RNP granules (to R.V.P.), and the National Institutes of Health (F32GM146418-01A1 to M.R.K. and R01NS121114 to R.V.P.). The contributions of J.M.L., A.E.P., and N.A.E. were supported, in part, by the Center for Biomolecular Condensates in the James F. McKelvey School of Engineering at Washington University in St. Louis. ; Cellular matter can be organized into compositionally distinct biomolecular condensates. For example, in Ashbya gossypii, the RNA-binding protein Whi3 forms distinct condensates with different RNA molecules. Using criteria derived from a physical framework for explaining how compositionally distinct condensates can form spontaneously via thermodynamic considerations, we find that condensates in vitro form mainly via heterotypic interactions in binary mixtures of Whi3 and RNA. However, within these condensates, RNA molecules become dynamically arrested. As a result, in ternary systems, simultaneous additions of Whi3 and pairs of distinct RNA molecules lead to well-mixed condensates, whereas delayed addition of an RNA component results in compositional distinctness. Therefore, compositional identities of condensates can be achieved via dynamical control, being driven, at least partially, by the dynamical arrest of RNA molecules. Finally, we show that synchronizing the production of different RNAs leads to more well-mixed, as opposed to compositionally distinct condensates in vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; text/xml; application/zip |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/361481Test |
| الإتاحة: | https://www.repository.cam.ac.uk/handle/1810/361481Test |
| رقم الانضمام: | edsbas.541239EE |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |