المؤلفون: Conley, C. A, Fritz-Six, K. L, Almenar-Queralt, A, Fowler, V. M

المصدر: Genomics. 73(2)

مصطلحات موضوعية: Life Sciences (General)

الوصف: The 64-kDa autoantigen D1 or 1D, first identified as a potential autoantigen in Graves' disease, is similar to the tropomodulin (Tmod) family of actin filament pointed end-capping proteins. A novel gene with significant similarity to the 64-kDa human autoantigen D1 has been cloned from both humans and mice, and the genomic sequences of both genes have been identified. These genes form a subfamily closely related to the Tmods and are here named the Leiomodins (Lmods). Both Lmod genes display a conserved intron-exon structure, as do three Tmod genes, but the intron-exon structure of the Lmods and the Tmods is divergent. mRNA expression analysis indicates that the gene formerly known as the 64-kDa autoantigen D1 is most highly expressed in a variety of human tissues that contain smooth muscle, earning it the name smooth muscle Leiomodin (SM-Lmod; HGMW-approved symbol LMOD1). Transcripts encoding the novel Lmod gene are present exclusively in fetal and adult heart and adult skeletal muscle, and it is here named cardiac Leiomodin (C-Lmod; HGMW-approved symbol LMOD2). Human C-Lmod is located near the hypertrophic cardiomyopathy locus CMH6 on human chromosome 7q3, potentially implicating it in this disease. Our data demonstrate that the Lmods are evolutionarily related and display tissue-specific patterns of expression distinct from, but overlapping with, the expression of Tmod isoforms. Copyright 2001 Academic Press.

الوصول الحر: https://ntrs.nasa.gov/citations/20040112474Test

المؤلفون: Conley, C. A, Fowler, V. M

المصدر: Current eye research. 19(4)

مصطلحات موضوعية: Life Sciences (General)

الوصف: PURPOSE. To evaluate the tissue-specific expression pattern of the 64kD human autoantigen D1, a tropomodulin-related protein that may be involved in thyroid-associated ophthalmopathy. METHODS. Recombinant 64kD human autoantigen D1 was generated in a bacterial expression system and used to immunize rabbits. Specific antibodies were affinity-purified and used for Western blots on normal and hyperthyroid rat and rabbit tissue, and immunofluorescence localization on cryosections of rat tissue. RESULTS. Anti-64kD human autoantigen D1 antibodies recognize specifically a approximately 70kD polypeptide in western blots of extraocular muscle, sternothyroid muscle, and smooth muscle. Immunofluorescence staining demonstrates that the 64kD human autoantigen D1 localizes to myofibrils in slow fibers from rat extraocular and sternothyroid muscle. The level of this protein is not altered in extraocular muscles from hyperthyroid rabbits. CONCLUSIONS. The 64kD human autoantigen D1 is expressed in slow fibers of extraocular and sternothyroid muscles as a component of myofibrils, and is not upregulated in conditions of hyperthyroidism.

الوصول الحر: https://ntrs.nasa.gov/citations/20040141799Test

المؤلفون: Fowler, V. J

مصطلحات موضوعية: Electronic Components And Circuits

الوصف: Sensor automatically and accurately measures the two-dimensional deflection angles of a laser beam to provide closed-loop servomechanism control of laser beam directivity.

الوصول الحر: https://ntrs.nasa.gov/citations/19680000481Test

المؤلفون: Fowler, V. J, Johnson, R, Kapuscienski, S, Schlafer, J, Weiss, P

مصطلحات موضوعية: Masers

الوصف: System for optical acquisition and tracking, and laser beam deflector devices - electro-optical techniques for controlling direction of laser beam flow

الوصول الحر: https://ntrs.nasa.gov/citations/19650020940Test

المؤلفون: Fowler, V. J, Johnson, R, Kapuscienski, S, Schlafer, J, Weiss, P

مصطلحات موضوعية: Masers

الوصف: Piezoelectrically powered laser beam waveguide for electro-optical acquisition and tracking

الوصول الحر: https://ntrs.nasa.gov/citations/19660008002Test