دورية أكاديمية
Protective effect of melatonin on Ca2+ homeostasis and contractility in acute cholecystitis
| العنوان: | Protective effect of melatonin on Ca2+ homeostasis and contractility in acute cholecystitis |
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| المؤلفون: | Gomez Pinilla, Pedro J, Camello, Pedro J, Pozo, María J |
| بيانات النشر: | Munksgaard International Publishers |
| سنة النشر: | 2008 |
| المجموعة: | KU Leuven: Lirias |
| مصطلحات موضوعية: | Acalculous Cholecystitis, Animals, Caffeine, Calcium, Cholecystitis, Acute, Common Bile Duct, Disease Models, Animal, Gallbladder, Guinea Pigs, Homeostasis, Ligation, Lipid Peroxidation, Male, Melatonin, Muscle Contraction, Nitrendipine, Pinacidil, Thapsigargin |
| الوصف: | Impaired Ca2+ homeostasis and smooth muscle contractility co-exist in acute cholecystitis (AC) leading to gallbladder dysfunction. There is no pharmacological treatment for this pathological condition. Our aim was to evaluate the effects of melatonin treatment on Ca2+ signaling pathways and contractility altered by cholecystitis. [Ca2+]i was determined by epifluorescence microscopy in fura-2 loaded isolated gallbladder smooth muscle cells, and isometric tension was recorded from gallbladder muscle strips. Malondialdehyde (MDA) and reduced glutathione (GSH) contents were determined by spectrophotometry and cycloxygenase-2 (COX-2) expression was quantified by western blot. Melatonin was tested in two experimental groups, one of which underwent common bile duct ligation for 2 days and another that was later de-ligated for 2 days. Inflammation-induced impairment of Ca2+ responses to cholecystokinin and caffeine were recovered by melatonin treatment (30 mg/kg). This treatment also ameliorated the detrimental effects of AC on Ca2+ influx through both L-type and capacitative Ca2+ channels, and it was effective in preserving the pharmacological phenotype of these channels. Despite its effects on Ca2+ homeostasis, melatonin did not improve contractility. After de-ligation, Ca2+ influx and contractility were still impaired, but both were recovered by melatonin. These effects of melatonin were associated to a reduction of MDA levels, an increase in GSH content and a decrease in COX-2 expression. These findings indicate that melatonin restores Ca2+ homeostasis during AC and resolves inflammation. In addition, this indoleamine helps in the subsequent recovery of functionality. ; status: published |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0742-3098 1600-079X |
| العلاقة: | Journal of Pineal Research vol:44 issue:3 pages:250-260; https://lirias.kuleuven.be/handle/123456789/280206Test; http://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0742-3098&date=2008&volume=44&issue=3&spage=250Test |
| الإتاحة: | https://lirias.kuleuven.be/handle/123456789/280206Test http://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0742-3098&date=2008&volume=44&issue=3&spage=250Test |
| رقم الانضمام: | edsbas.5C305E87 |
| قاعدة البيانات: | BASE |
| تدمد: | 07423098 1600079X |
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