دورية أكاديمية
Patterns of Differentially Expressed circRNAs in Human Thymocytes
| العنوان: | Patterns of Differentially Expressed circRNAs in Human Thymocytes |
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| المؤلفون: | López-Nieva, Pilar, Fernandez-Navarro, Pablo L, Cobos-Fernández, María Ángeles, González-Vasconcellos, Iria, Sánchez Pérez, Raúl, Aroca, Ángel, Fernández-Piqueras, José, Santos, Javier |
| المساهمون: | Ministerio de Ciencia, Innovación y Universidades (España), Fundación Ramón Areces, Comunidad de Madrid, Asociación Española Contra el Cáncer, Banco Santander, Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute (MDPI) |
| سنة النشر: | 2022 |
| المجموعة: | REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
| مصطلحات موضوعية: | CircRNAs, Human thymocytes, CircRNAm-iRNA-mRNA controlling networks |
| الوصف: | Circular RNAs (circRNAs) are suggested to play a discriminative role between some stages of thymocyte differentiation. However, differential aspects of the stage of mature single-positive thymocytes remain to be explored. The purpose of this study is to investigate the differential expression pattern of circRNAs in three different development stages of human thymocytes, including mature single-positive cells, and perform predictions in silico regarding the ability of specific circRNAs when controlling the expression of genes involved in thymocyte differentiation. We isolate human thymocytes at three different stages of intrathymic differentiation and determine the expression of circRNAs and mRNA by RNASeq. We show that the differential expression pattern of 50 specific circRNAs serves to discriminate between the three human thymocyte populations. Interestingly, the downregulation of RAG2, a gene involved in T-cell differentiation in the thymus, could be simultaneously controlled by the downregulation of two circRNASs (hsa_circ_0031584 and hsa_circ_0019079) through the hypothetical liberation of hsa-miR-609. Our study provides, for the first time, significant insights into the usefulness of circRNAs in discriminating between different stages of thymocyte differentiation and provides new potential circRNA-miRNA-mRNA networks capable of controlling the expression of genes involved in T-cell differentiation in the thymus. ; This work was financed by grants from the Spanish Ministry of Science, Innovation and Universities (MCIU)(RTI2018- 093330-B-I00; MCIU/FEDER, EU), Ramón Areces Foundation (CIVP19S7917); Autonomous Community of Madrid, Spain (B2017/BMD-3778; LINFOMAS-CM); the Spanish Association Against Cancer (AECC, 2018; PROYE18054PIRI); and the Spanish Ministry (Juan de la Cierva Grant IJCI-2016-29155). Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. ; Sí |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2311-553X |
| العلاقة: | https://doi.org/10.3390/ncrna8020026Test; info:eu-repo/grantAgreement/ES/IJCI-2016-29155; info:eu-repo/grantAgreement/ES/RTI2018-093330-B-I00; Noncoding RNA. 2022 Mar 30;8(2):26.; http://hdl.handle.net/20.500.12105/14852Test; Non-coding RNA |
| DOI: | 10.3390/ncrna8020026 |
| الإتاحة: | https://doi.org/20.500.12105/14852Test https://doi.org/10.3390/ncrna8020026Test https://hdl.handle.net/20.500.12105/14852Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; Atribución 4.0 Internacional ; open access |
| رقم الانضمام: | edsbas.172F9C6F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2311553X |
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| DOI: | 10.3390/ncrna8020026 |