دورية أكاديمية

A Single Oral Immunization with Replication-Competent Adenovirus-Vectored Vaccine Induces a Neutralizing Antibody Response in Mice against Canine Distemper Virus.

التفاصيل البيبلوغرافية
العنوان: A Single Oral Immunization with Replication-Competent Adenovirus-Vectored Vaccine Induces a Neutralizing Antibody Response in Mice against Canine Distemper Virus.
المؤلفون: Du, Xiang, Goffin, Emeline, Gillard, Lucie, Machiels, Bénédicte, Gillet, Laurent
المصدر: Viruses, 14 (9) (2022-08-23)
بيانات النشر: Multidisciplinary Digital Publishing Institute (MDPI), 2022.
سنة النشر: 2022
مصطلحات موضوعية: Adenovirus Vaccines, Antibodies, Neutralizing, Antibodies, Viral, Viral Vaccines, Adenoviridae/genetics, Adenoviridae Infections, Animals, Distemper/prevention & control, Distemper Virus, Canine/physiology, Dogs, Humans, Immunization, Mice, Mice, Inbred BALB C, Vaccination, canine distemper virus, morbillivirus, oral vaccine, replicative adenovirus vector, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale
الوصف: Canine Distemper Virus (CDV) is a fatal and highly contagious pathogen of multiple carnivores. While injectable vaccines are very effective in protecting domestic animals, their use in the wild is unrealistic. Alternative vaccines are therefore needed. Adenovirus (AdV) vectors are popular vaccine vectors due to their capacity to elicit potent humoral and cellular immune responses against the antigens they carry. In parallel, vaccines based on live human AdV-4 and -7 have been used in U.S. army for several decades as replicative oral vaccines against respiratory infection with the same viruses. Based on these observations, the use of oral administration of replication competent AdV-vectored vaccines has emerged as a promising tool especially for wildlife vaccination. Developing this type of vaccine is not easy, however, given the high host specificity of AdVs and their very low replication in non-target species. To overcome this problem, the feasibility of this approach was tested using mouse adenovirus 1 (MAV-1) in mice as vaccine vectors. First, different vaccine vectors expressing the entire or part H or F proteins of CDV were constructed. These different strains were then used as oral vaccines in BALB/c mice and the immune response to CDV was evaluated. Only the strain expressing the full length CDV H protein generated a detectable and neutralizing immune response to CDV. Secondly, using this strain, we were able to show that although this type of vaccine is sensitive to pre-existing immunity to the vector, a second oral administration of the same vaccine is able to boost the immune response against CDV. Overall, this study demonstrates the feasibility of using replicating AdVs as oral vaccine vectors to immunize against CDV in wildlife carnivores.
نوع الوثيقة: journal article
http://purl.org/coar/resource_type/c_6501Test
article
peer reviewed
اللغة: English
العلاقة: urn:issn:1999-4915
DOI: 10.3390/v14091847
الوصول الحر: https://orbi.uliege.be/handle/2268/301172Test
حقوق: open access
http://purl.org/coar/access_right/c_abf2Test
info:eu-repo/semantics/openAccess
رقم الانضمام: edsorb.301172
قاعدة البيانات: ORBi