دورية أكاديمية
Characterization of an immunodominant epitope in the endodomain of the coronavirus membrane protein
| العنوان: | Characterization of an immunodominant epitope in the endodomain of the coronavirus membrane protein |
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| المؤلفون: | Dong, Hui, Zhang, X., Shi, H., Chen, J., Da, S., Zhu, Y., Li, F. |
| المصدر: | Viruses, 8 (12) (2016) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute (MDPI) |
| سنة النشر: | 2016 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Coronavirus, Endodomain, Immunodominant epitope, Membrane protein, M protein, Article, Western blotting, Bagg albino mouse, Transmissible gastroenteritis virus, Animals, Antibodies, Monoclonal, Viral, Blotting, Western, Cell Line, Epitope Mapping, Fluorescent Antibody Technique, Immunodominant Epitopes, Mice, Inbred BALB C, Swine, Viral Matrix Proteins, Life sciences, Veterinary medicine & animal health, Sciences du vivant, Médecine vétérinaire & santé animale |
| الوصف: | peer reviewed ; The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication. © 2016 by the authors; licensee MDPI, Basel, Switzerland. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1999-4915 |
| العلاقة: | urn:issn:1999-4915; https://orbi.uliege.be/handle/2268/254416Test; info:hdl:2268/254416; scopus-id:2-s2.0-85007070773; info:pmid:27973413 |
| DOI: | 10.3390/v8120327 |
| الإتاحة: | https://doi.org/10.3390/v8120327Test https://orbi.uliege.be/handle/2268/254416Test |
| حقوق: | restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.5DB65303 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19994915 |
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| DOI: | 10.3390/v8120327 |