Antiviral, Immunomodulatory and Antiproliferative Activities of Recombinant Soluble IFNAR2 without IFN-ß Mediation
| العنوان: | Antiviral, Immunomodulatory and Antiproliferative Activities of Recombinant Soluble IFNAR2 without IFN-ß Mediation |
|---|---|
| المؤلفون: | Shohreh Issazadeh-Navikas, Isaac Hurtado-Guerrero, José Pavia, María Jesús Pinto-Medel, Patricia Urbaneja, Natalia Mena-Vázquez, A. Alonso, Begoña Oliver-Martos, José Alcamí, Esther Calonge, Oscar Fernández, Laura Leyva, Antonio Alcami, Pablo Aliaga, Jose Luis Rodriguez-Bada, Bruno Hernáez |
| المساهمون: | Instituto de Salud Carlos III, Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Regional Government of Andalusia (España), Red Española de Esclerosis Múltiple, Junta de Andalucía, European Commission |
| المصدر: | Journal of Clinical Medicine Volume 9 Issue 4 Digital.CSIC. Repositorio Institucional del CSIC instname Dipòsit Digital de la UB Universidad de Barcelona Journal of Clinical Medicine, Vol 9, Iss 959, p 959 (2020) Hurtado-guerrero, I, Hernáez, B, Pinto-medel, M J, Calonge, E, Rodriguez-bada, J L, Urbaneja, P, Alonso, A, Mena-vázquez, N, Aliaga, P, Issazadeh-navikas, S, Pavia, J, Leyva, L, Alcamí, J, Alcamí, A, Fernández, Ó & Oliver-martos, B 2020, ' Antiviral, Immunomodulatory and Antiproliferative Activities of Recombinant Soluble IFNAR2 without IFN-ß Mediation ', Journal of Clinical Medicine, vol. 9, no. 4, pp. 959 . https://doi.org/10.3390/jcm9040959Test Repisalud Instituto de Salud Carlos III (ISCIII) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute (MDPI), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_treatment, soluble receptors, Cell, Immunology, lcsh:Medicine, Interferó, IFNAR, Pharmacology, Virus, Article, law.invention, immunology, 03 medical and health sciences, 0302 clinical medicine, Immune system, law, Interferon, medicine, Immunologia, Receptor, Soluble receptors, business.industry, lcsh:R, General Medicine, interferon, 030104 developmental biology, medicine.anatomical_structure, Cytokine, Recombinant DNA, Signal transduction, business, 030217 neurology & neurosurgery, medicine.drug |
| الوصف: | Soluble receptors of cytokines are able to modify cytokine activities and therefore the immune system, and some have intrinsic biological activities without mediation from their cytokines. The soluble interferon beta (IFN-ß ) receptor is generated through alternative splicing of IFNAR2 and has both agonist and antagonist properties for IFN-ß but its role is unknown. We previously demonstrated that a recombinant human soluble IFN-ß receptor showed intrinsic therapeutic efficacy in a mouse model of multiple sclerosis. Here we evaluate the potential biological activities of recombinant sIFNAR2 without the mediation of IFN-ß in human cells. Recombinant sIFNAR2 down-regulated the production of IL-17 and IFN-ɣ and reduced the cell proliferation rate. Moreover, it showed a strong antiviral activity, fully protecting the cell monolayer after being infected by the virus. Specific inhibitors completely abrogated the antiviral activity of IFN-ß but not that of the recombinant sIFNAR2, and there was no activation of the JAK-STAT signaling pathway. Consequently, r-sIFNAR2 exerts immunomodulatory, antiproliferative and antiviral activities without IFN-ß mediation, and could be a promising treatment against viral infections and immune-mediated diseases. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0674109f235fe62e9bedb2c4821b8dcbTest https://hdl.handle.net/20.500.12105/9486Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0674109f235fe62e9bedb2c4821b8dcb |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |