التفاصيل البيبلوغرافية
| العنوان: |
The RNA Capping Enzyme Domain in Protein A is Essential for Flock House Virus Replication |
| المؤلفون: |
Tania Quirin, Yu Chen, Maija K. Pietilä, Deyin Guo, Tero Ahola |
| المصدر: |
Viruses; Volume 10; Issue 9; Pages: 483 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2018 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
RNA replication, nodavirus, alphavirus, RNA capping enzyme, replication complex |
| جغرافية الموضوع: |
agris |
| الوصف: |
The nodavirus flock house virus (FHV) and the alphavirus Semliki Forest virus (SFV) show evolutionarily intriguing similarities in their replication complexes and RNA capping enzymes. In this study, we first established an efficient FHV trans-replication system in mammalian cells, which disjoins protein expression from viral RNA synthesis. Following transfection, FHV replicase protein A was associated with mitochondria, whose outer surface displayed pouch-like invaginations with a ‘neck’ structure opening towards the cytoplasm. In mitochondrial pellets from transfected cells, high-level synthesis of both genomic and subgenomic RNA was detected in vitro and the newly synthesized RNA was of positive polarity. Secondly, we initiated the study of the putative RNA capping enzyme domain in protein A by mutating the conserved amino acids H93, R100, D141, and W215. RNA replication was abolished for all mutants inside cells and in vitro except for W215A, which showed reduced replication. Transfection of capped RNA template did not rescue the replication activity of the mutants. Comparing the efficiency of SFV and FHV trans-replication systems, the FHV system appeared to produce more RNA. Using fluorescent marker proteins, we demonstrated that both systems could replicate in the same cell. This work may facilitate the comparative analysis of FHV and SFV replication. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Insect Viruses; https://dx.doi.org/10.3390/v10090483Test |
| DOI: |
10.3390/v10090483 |
| الإتاحة: |
https://doi.org/10.3390/v10090483Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.EA21CB3A |
| قاعدة البيانات: |
BASE |
