Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines
| العنوان: | Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines |
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| المؤلفون: | Klaus D. Feussner, Miriam A. Dam, Jioji N. Tabudravu, Luis M. Botana, Marcel Jaspars, Mostafa E. Rateb, Rebeca Alvariño, Marta Leirós, Eva Alonso |
| المساهمون: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
| المصدر: | Marine Drugs; Volume 14; Issue 11; Pages: 197 Marine Drugs Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Marine Drugs, Vol 14, Iss 11, p 197 (2016) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Antioxidant, F163, medicine.medical_treatment, neurons, Pharmaceutical Science, Quinolones, Mitochondrion, medicine.disease_cause, Antioxidants, Mice, chemistry.chemical_compound, Pregnancy, Drug Discovery, oxidative stress, nrf2, lcsh:QH301-705.5, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Neurons, Marine sponge, chemistry.chemical_classification, biology, Glutathione, Mitochondria, Porifera, 3. Good health, Neuroprotective Agents, Biochemistry, Catalase, Female, Zyzzya, marine sponge, NF-E2-Related Factor 2, Primary Cell Culture, Article, Cell Line, Structure-Activity Relationship, 03 medical and health sciences, Investigación::32 Ciencias médicas::3209 Farmacología [Materias], makaluvamine, Cell Line, Tumor, medicine, Animals, Structure–activity relationship, Pyrroles, Pyrroloiminoquinones, Reactive oxygen species, Activator (genetics), Hydrogen Peroxide, 030104 developmental biology, lcsh:Biology (General), chemistry, Oxidative stress, biology.protein, Reactive Oxygen Species, Makaluvamine |
| الوصف: | Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H2O2. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correctmitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p-hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells. The research leading to these results received funding from the following FEDER cofounded-grants. From CDTI and Technological Funds, supported by the Ministerio de Economía y Competitividad, AGL2012-40185-CO2-01, AGL2014-58210-R, and the Consellería de Cultura, Educación e Ordenación Universitaria, GRC2013-016, and through the Axencia Galega de Innovación, Spain, ITC-20133020 SINTOX. From CDTI under ISIP Programme, Spain, IDI-20130304 APTAFOOD. From the European Union’s Seventh Framework Programme managed by REA—Research Executive Agency (FP7/2007-2013) under grant agreement 312184 PHARMASEA. SI |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1660-3397 |
| DOI: | 10.3390/md14110197 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a360b05621321b62be6a8551c8c8b6a9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a360b05621321b62be6a8551c8c8b6a9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
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| DOI: | 10.3390/md14110197 |