المؤلفون: I. Jolanda M. de Vries, Sofie H. Tolmeijer, Rutger H. T. Koornstra, Niven Mehra, Sandra van Wilpe

المصدر: Immuno
Volume 1
Issue 2
Pages 5-77
Immuno, Vol 1, Iss 5, Pp 67-77 (2021)

مصطلحات موضوعية: 0301 basic medicine, Oncology, medicine.medical_specialty, medicine.medical_treatment, Ipilimumab, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Downregulation and upregulation, Internal medicine, Lactate dehydrogenase, medicine, melanoma, Distribution (pharmacology), business.industry, Melanoma, biomarkers, lactate dehydrogenase, Immunotherapy, medicine.disease, Isotype, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, Medicine, Nivolumab, business, checkpoint inhibitors, medicine.drug

الوصف: Serum lactate dehydrogenase (LDH) levels are inversely related with response to immune checkpoint inhibitors (ICIs) in patients with metastatic melanoma. LDH is a key regulator of glycolysis, a pathway known to be upregulated in malignant tumors and to negatively affect antitumor immunity. We hypothesized that LDH isotype distribution in peripheral blood better reflects tumor glycolytic activity than total LDH levels and might therefore contribute to immunotherapy response prediction. LDH isotyping was performed in blood of 40 patients with metastatic melanoma and elevated LDH levels, of which 22 were treated with ipilimumab plus nivolumab. LDH-1 levels were decreased in 57.5% of patients. The percentage of LDH-2, -3 and -4, on the other hand, was elevated in 35%, 67.5% and 37.5% of patients, respectively. There was no difference in LDH isotype distribution between patients with versus patients without clinical benefit of ICIs, except for a numerically lower percentage of LDH-1 in patients without clinical benefit (median 13.3% vs. 17.6%, p = 0.1295). The percentage of LDH-1 correlated with total LDH levels and tumor burden and is therefore not likely to have strong, independent predictive value for response to ICIs. In conclusion, LDH isotyping does not contribute to ICI response prediction in melanoma patients with elevated LDH levels.

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المؤلفون: Liliana Burlibasa, Irina Adriana Beuran, Dragos Mihai Guita, Alexandru Petre, Sergiu Drafta, Mihai Burlibasa, Corina Marilena Cristache

المصدر: Applied Sciences
Volume 11
Issue 22
Applied Sciences, Vol 11, Iss 11012, p 11012 (2021)

مصطلحات موضوعية: marginal bone loss, medicine.medical_specialty, Saliva, Technology, total antioxidant status, QH301-705.5, medicine.medical_treatment, QC1-999, Gastroenterology, Proinflammatory cytokine, chemistry.chemical_compound, Internal medicine, Lactate dehydrogenase, medicine, General Materials Science, Interleukin 8, Biology (General), Dental implant, Interleukin 6, Instrumentation, QD1-999, Fluid Flow and Transfer Processes, biology, business.industry, Process Chemistry and Technology, Physics, General Engineering, periimplantitis, Engineering (General). Civil engineering (General), Computer Science Applications, Antioxidant capacity, Chemistry, salivary biomarker, chemistry, biology.protein, Tumor necrosis factor alpha, TA1-2040, business

الوصف: Background: The mechanisms of periimplantitis are mostly based on biofilm-induced inflammatory lesions around dental implants. However, the host reaction, reflected by the intensity of the inflammatory response to bacterial products, is crucial for peri-implant bone destruction. The aim of this pilot study was to measure total antioxidant status (TAS), the amount of IL-6, IL-8 and TNF-α (tumor necrosis factor), salivary lactate dehydrogenase (LDH) levels and the correlation with one-year peri-implant bone loss. Methods: Seven consecutive patients (Group 1), with the presence of at least one prior inserted dental implant, and three healthy individuals, fully dentate (Group 0), were enrolled. IL-6, IL-8, TNF-α, TAS and LDH were determined in saliva of both groups. Peri-implant marginal bone loss (MBL) at one year compared to baseline was evaluated for Group 1. Results: A statistically significant positive correlation was found in Group 1 between MBL and the IL-8 (p = 0.033) and a strong negative correlation between MBL and TAS (p = 0.002), but no statistically significant differences of the assessed parameters were found between the two groups. Conclusions: In the limits of the present preliminary study, salivary TAS and proinflammatory cytokines could be associated with the risk of peri-implant bone loss over time.

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المؤلفون: Taslim Ersam, Edwin Risky Sukandar, Johanis Wairata, Arif Fadlan, Muhammad Taher, Adi Setyo Purnomo

المصدر: Biomedicines
Volume 9
Issue 10
Biomedicines, Vol 9, Iss 1380, p 1380 (2021)

مصطلحات موضوعية: Antioxidant, antioxidant, DPPH, QH301-705.5, medicine.medical_treatment, Medicine (miscellaneous), Garcinia forbesii, General Biochemistry, Genetics and Molecular Biology, Article, law.invention, chemistry.chemical_compound, law, Lactate dehydrogenase, antiplasmodial, medicine, Biology (General), xanthones, chemistry.chemical_classification, ABTS, biology, Traditional medicine, antidiabetic, Plasmodium falciparum, molecular docking, biology.organism_classification, In vitro, Enzyme, chemistry, Phytotherapy

الوصف: This study aimed to isolate xanthones from Garcinia forbesii and evaluated their activity in vitro and in silico. The isolated compounds were evaluated for their antioxidant activity by DPPH, ABTS and FRAP methods. The antidiabetic activity was performed against α-glucosidase and α-amylase enzymes. The antiplasmodial activity was evaluated using Plasmodium falciparum strain 3D7 sensitive to chloroquine. Molecular docking analysis on the human lysosomal acid-alpha-glucosidase enzyme (5NN8) and P. falciparum lactate dehydrogenase enzyme (1CET) and prediction of ADMET for the active compound, were also studied. For the first time, lichexanthone (1), subelliptenone H (2), 12b-hydroxy-des-D-garcigerrin A (3), garciniaxanthone B (4) and garcigerin A (5) were isolated from the CH2Cl2 extract of the stem bark of G. forbesii. Four xanthones (Compounds 2–5) showed strong antioxidant activity. In vitro α-glucosidase test showed that Compounds 2 and 5 were more active than the others, while Compound 4 was the strongest against α-amylase enzymes. In vitro antiplasmodial evaluation revealed that Compounds 2 and 3 showed inhibitory activity on P. falciparum. Molecular docking studies confirmed in vitro activity. ADMET predictions suggested that Compounds 1–5 were potential candidates for oral drugs. The isolated 2–5 can be used as promising phytotherapy in antidiabetic and antiplasmodial treatment.

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المؤلفون: Ahnryul Choi, Hyok-ju Eum, Sagn-Jin Kim, Woon-Seob Shin, Sangsik Lee, Nam-Ik Kim, Jee-Hun Jang, Buom Kim

المصدر: Applied Sciences
Volume 10
Issue 23
Applied Sciences, Vol 10, Iss 8383, p 8383 (2020)

مصطلحات موضوعية: 0209 industrial biotechnology, medicine.medical_specialty, medicine.medical_treatment, Entire upper body, 02 engineering and technology, blood test, Muscle damage, lcsh:Technology, creatinine kinase, fatigue rehabilitation, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, 020901 industrial engineering & automation, 0302 clinical medicine, Internal medicine, Thalassotherapy, Lactate dehydrogenase, medicine, Blood test, General Materials Science, lcsh:QH301-705.5, Instrumentation, Fluid Flow and Transfer Processes, chemistry.chemical_classification, medicine.diagnostic_test, biology, Muscle fatigue, lcsh:T, business.industry, Process Chemistry and Technology, General Engineering, lactate dehydrogenase, 030229 sport sciences, lcsh:QC1-999, thalassotherapy adverse effects, Computer Science Applications, Endocrinology, Enzyme, lcsh:Biology (General), lcsh:QD1-999, chemistry, lcsh:TA1-2040, biology.protein, Creatine kinase, lcsh:Engineering (General). Civil engineering (General), business, lcsh:Physics

الوصف: The purpose of this study was to verify the effect of deep-sea water thalassotherapy (DSWTT) on recovery from fatigue and muscle damage. The same exercise program is conducted in general underwater and deep-sea water to confirm the characteristics of deep-sea water through fatigue recovery and muscle damage enzymes. A total of 30 male college students were studied, including 10 belonging to the control group (CG), 10 in the water exercise group (WEG), and 10 in the deep-sea water exercise group (DSWEG). The DSWTT treatment consists of three components&mdash
preheating, treatment, and cooling&mdash
and the DSWTT program stretches and massages the entire upper body, lower body, back, and the entire body for a total of 25 min in a deep-sea tank. After the DSWTT program, blood tests were conducted to confirm the level of fatigue-related parameters and muscle damage enzymes. Fatigue-related parameters including glucose, lactate, ammonia, and lactate dehydrogenase (LDH), and the levels of muscle damage enzymes such as creatinine kinase (CK) and aspartate aminotransferase (AST) were measured. The results revealed that fatigue had a primary effect (p <
0.001) and exhibited strongly significant interaction (p <
0.001) with lactate, ammonia, and LDH levels, whereas the glucose level remained unchanged. The post hoc results showed a significant decrease in these parameters among DSWEG compared to CG and WEG (p <
0.01). Muscle damage enzymes showed a main effect (p <
0.001) and significant interaction (p <
0.001) with CK and AST (p <
0.001). The post hoc results showed a significant decrease in DSWEG compared with CG and WEG (p <
0.01). In conclusion, the DSWTT program applied to this study showed significant effects on muscle fatigue and muscle damage recovery. When the DSWTT program is applied in hot springs, it can have a positive effect on muscle fatigue and muscle damage recovery and can contribute to improving national health and quality of life. Further studies are needed to investigate DSWTT programs with various research subjects at different program temperatures, exercise times, and frequencies of treatment and exercise.

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المؤلفون: Mahyar Zare, Markéta Prokešová, Eliška Kučerová, Hung Quang Tran, Anna Pavlovna Ivanova, Tatyana Gebauer, Vlastimil Stejskal, Milena Bušová

المصدر: Animals
Volume 11
Issue 8
Animals, Vol 11, Iss 2266, p 2266 (2021)

مصطلحات موضوعية: Clarias gariepinus, Antioxidant, Veterinary medicine, medicine.medical_treatment, Feed additive, 010501 environmental sciences, 01 natural sciences, chemistry.chemical_compound, Lactate dehydrogenase, SF600-1100, medicine, Leonardite, Food science, glutathione, Clariidae, 0105 earth and related environmental sciences, General Veterinary, biology, Cholesterol, fish nutrition, 04 agricultural and veterinary sciences, Glutathione, biology.organism_classification, Siberian leonardite, QL1-991, chemistry, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Animal Science and Zoology, biochemical parameters, Zoology, Catfish

الوصف: In the present study, a possible nature immunostimulant and growth promoter—humic substances (HS) originating from Siberian leonardite mineraloid—were tested on juvenile Clarias gariepinus performance. Feed additive was applied onto commercial pelleted feed at four HS levels—0, 1, 3, and 6% w/w (HS0, HS1, HS3, HS6, respectively). Diets were tested in five repetitions (in total, n = 1800 individuals, mean body weight 28.1 ± 6.2 g) for 56 days. Growth and production parameters, fish condition and somatic indices, and overall mortality were evaluated after 14, 28, 42, and 56 days of exposure. Whereas, plasma samples were collected only after 0, 28, and 56 days, when fish health status was assessed with biochemical parameters (total proteins, TP
alanine aminotransferase, ALT
aspartate aminotransferase, AST
lactate dehydrogenase, LDH
cholesterol, CHOL
triglycerides, TAG) and fish antioxidant status with glutathione (reduced glutathione, GSH
oxidized glutathione, GSSG
glutathione ratio GSH/GSSG). Although a significantly positive effect of HS feed additive on growth performance was not found in the present study, moderately positive effects were found regarding biochemical parameters (ALT, AST, LDH, CHOL, and TAG) and antioxidants (GSH/GSSG ratio) that were improved especially in the HS3 group.

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المؤلفون: Nan Zhang, Shunshan Jiao, Pu Jing

المصدر: Antioxidants
Volume 10
Issue 6
Antioxidants, Vol 10, Iss 930, p 930 (2021)

مصطلحات موضوعية: 0301 basic medicine, Antioxidant, Physiology, medicine.medical_treatment, Clinical Biochemistry, Oxidative phosphorylation, RM1-950, medicine.disease_cause, Biochemistry, Article, HSP-1 pathway, 03 medical and health sciences, chemistry.chemical_compound, food, Lactate dehydrogenase, medicine, Viability assay, Molecular Biology, 030109 nutrition & dietetics, Red cabbage, biology, caco-2 cells, Chemistry, Cell Biology, Ascorbic acid, phytochemicals, anthocyanins, food.food, Cell biology, CaMKII pathway, 030104 developmental biology, Sirtuin, biology.protein, Therapeutics. Pharmacology, Oxidative stress, lifespan

الوصف: Many studies have demonstrated that cabbages possess various biological activities, and our previous studies confirmed that cyanidin-3-diglucoside-5-glucoside (CY3D5G), the major core of red cabbage anthocyanins, exhibited in vitro antioxidant activity. This study further investigated the protective effects of CY3D5G derivative from red cabbage juice (RCJ) on oxidative stress and lifespan in cells and Caenorhabditis elegans, green cabbage juice (GCJ) was used as control. RCJ rather than GCJ significantly improved cell viability and decreased lactate dehydrogenase release in H2O2-induced caco-2 cells. RCJ significantly increased survival during oxidative and heat stress and mean lifespan in C. elegans by 171.63% and 31.64%, and 28.16%, respectively, while GCJ treatment showed no significant effects (p <
0.05). These results might be attributed to significantly (p <
0.05) higher contents of total phenolics, ascorbic acid, glucosinolates, and anthocyanins in RCJ compared to those in GCJ. Additionally, both of them decreased autofluorescence and reproductive capacity, increased body length, but did not alter the intracellular ROS level. Prolonged lifespan by RCJ might require heat-shock transcription factor pathway, sirtuin signaling, and calmodulin kinase II pathway, independent of insulin/insulin-like growth factor-1 signaling pathway. RCJ showed promising antioxidant properties in caco-2 cells and C. elegans, which provided more information on the health benefits of cabbage.

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المؤلفون: Andreas Lendlein, F. Jung, Christian Schulz, Anne Krüger-Genge, Jan-Heiner Küpper

المساهمون: Publica

المصدر: International Journal of Molecular Sciences
Volume 22
Issue 3
International Journal of Molecular Sciences, Vol 22, Iss 1493, p 1493 (2021)
Schulz, C.; Krüger-Genge, A.; Lendlein, A.; Küpper, J.; Jung, F.: Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture. In: International Journal of Molecular Sciences. Vol. 22 (2021) 3, 1493. (DOI: /10.3390/ijms22031493)

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_treatment, nonadherent, Cell, Cell Culture Techniques, Prostacyclin, Apoptosis, 030204 cardiovascular system & hematology, lcsh:Chemistry, 0302 clinical medicine, lcsh:QH301-705.5, Spectroscopy, Cell Death, Chemistry, Biomaterial, General Medicine, Adhesion, Recombinant Proteins, Computer Science Applications, Cell biology, medicine.anatomical_structure, human venous endothelial cells, cardiovascular system, Cytokines, Intercellular Signaling Peptides and Proteins, Inflammation Mediators, Cell Division, medicine.drug, Surface Properties, Context (language use), adherent, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Thromboxane A2, medicine, Cell Adhesion, Human Umbilical Vein Endothelial Cells, Humans, Physical and Theoretical Chemistry, Molecular Biology, L-Lactate Dehydrogenase, Tumor Necrosis Factor-alpha, Growth factor, viability, Organic Chemistry, mediator release, Epoprostenol, In vitro, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Culture Media, Conditioned, Polystyrenes

الوصف: The adherence and shear-resistance of human umbilical venous endothelial cells (HUVEC) on polymers is determined in vitro in order to qualify cardiovascular implant materials. In these tests, variable fractions of HUVEC do not adhere to the material but remain suspended in the culture medium. Nonadherent HUVEC usually stop growing, rapidly lose their viability and can release mediators able to influence the growth and function of the adherent HUVEC. The aim of this study was the investigation of the time dependent behaviour of HUVEC under controlled nonadherent conditions, in order to gain insights into potential influences of these cells on their surrounding environment in particular adherent HUVEC in the context of in vitro biofunctionality assessment of cardiovascular implant materials. Data from adherent or nonadherent HUVEC growing on polystyrene-based cell adhesive tissue culture plates (TCP) or nonadhesive low attachment plates (LAP) allow to calculate the number of mediators released into the culture medium either from adherent or nonadherent cells. Thus, the source of the inflammatory mediators can be identified. For nonadherent HUVEC, a time-dependent aggregation without further proliferation was observed. The rate of apoptotic/dead HUVEC progressively increased over 90% within two days. Concomitant with distinct blebbing and loss of membrane integrity over time, augmented releases of prostacyclin (PGI2, up to 2.91 ± 0.62 fg/cell) and platelet-derived growth factor BB (PDGF-BB, up to 1.46 ± 0.42 fg/cell) were detected. The study revealed that nonadherent, dying HUVEC released mediators, which can influence the surrounding microenvironment and thereby the results of in vitro biofunctionality assessment of cardiovascular implant materials. Neglecting nonadherent HUVEC bears the risk for under- or overestimation of the materials endothelialization potential, which could lead to the loss of relevant candidates or to uncertainty with regard to their suitability for cardiac applications. One approach to minimize the influence from nonadherent endothelial cells could be their removal shortly after observing initial cell adhesion. However, this would require an individual adaptation of the study design, depending on the properties of the biomaterial used.

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المؤلفون: M Marzouq Al Ahbabi, Niraj Kumar Jha, Shreesh Ojha, M.F. Nagoor Meeran, Sheikh Azimullah, Sameer N. Goyal, Vinoth-Kumar Lakshmanan

المصدر: Molecules
Volume 25
Issue 23
Molecules, Vol 25, Iss 5656, p 5656 (2020)

مصطلحات موضوعية: Male, 0301 basic medicine, sesquiterpene, Cardiotonic Agents, Antioxidant, natural products, medicine.medical_treatment, Myocardial Infarction, Pharmaceutical Science, Pharmacology, medicine.disease_cause, Antioxidants, Article, Analytical Chemistry, lcsh:QD241-441, bioactive agents, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, lcsh:Organic chemistry, In vivo, Lactate dehydrogenase, Drug Discovery, medicine, Animals, Oil Red O, Rats, Wistar, Physical and Theoretical Chemistry, essential oils, Dyslipidemias, Polycyclic Sesquiterpenes, Organic Chemistry, Lipid metabolism, medicine.disease, Lipids, Bioactive compound, Rats, Oxidative Stress, 030104 developmental biology, chemistry, Chemistry (miscellaneous), 030220 oncology & carcinogenesis, Molecular Medicine, nootkatone, fragrant molecules, Lipid Peroxidation, Oxidative stress, Dyslipidemia

الوصف: In the present study, we assessed whether nootkatone (NKT), a sesquiterpene in edible plants, can provide protection against dyslipidemia, intramyocardial lipid accumulation, and altered lipid metabolism in a rat model of myocardial infarction (MI) induced by subcutaneous injections of isoproterenol (ISO, 85 mg/kg) on days 9 and 10. The rats were pre- and co-treated with NKT (10 mg/kg, p.o.) administered daily for 11 days. A significant reduction in the activities of myocardial creatine kinase and lactate dehydrogenase, as well as non-enzymatic antioxidants, and alterations in lipids and lipoproteins, along with a rise in plasma lipid peroxidation and intramyocardial lipid accumulation, were observed in ISO-treated rats. ISO administration induced alterations in the activities of enzymes/expressions that played a significant role in altering lipid metabolism. However, NKT treatment favorably modulated all biochemical and molecular parameters altered by ISO and showed protective effects against oxidative stress, dyslipidemia, and altered lipid metabolism, attributed to its free-radical-scavenging and antihyperlipidemic activities in rats with ISO-induced MI. Additionally, NKT decreased the accumulation of lipids in the myocardium as evidenced from Oil red O staining. Furthermore, the in vitro observations demonstrate the potent antioxidant property of NKT. The present study findings are suggestive of the protective effects of NKT on dyslipidemia and the underlying mechanisms. Based on our findings, it can be suggested that NKT or plants rich in NKT can be promising for use as a phytopharmaceutical or nutraceutical in protecting the heart and correcting lipid abnormalities and dyslipidemia, which are risk factors for ischemic heart diseases.

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المؤلفون: Miran Jang, Gun Hee Kim, Kee-Hong Kim

المصدر: Antioxidants
Volume 9
Issue 3
Antioxidants, Vol 9, Iss 3, p 200 (2020)
Department of Food Science Faculty Publications

مصطلحات موضوعية: 0301 basic medicine, antioxidant, Antioxidant, food.ingredient, Physiology, medicine.medical_treatment, Clinical Biochemistry, phenolic compounds, thistle (Cirsium japonicum), reactive oxygen species (ROS), PC 12 cells, Caenorhabditis elegans, Biochemistry, Article, 03 medical and health sciences, chemistry.chemical_compound, food, Chlorogenic acid, Lactate dehydrogenase, medicine, Food science, Viability assay, Molecular Biology, thistle (Cirsium japonicum), phenolic compounds, antioxidant, reactive oxygen species (ROS), PC 12 cells, Caenorhabditis elegans, chemistry.chemical_classification, Reactive oxygen species, 030109 nutrition & dietetics, biology, Chemistry, lcsh:RM1-950, Pectolinarin, Cell Biology, biology.organism_classification, lcsh:Therapeutics. Pharmacology, 030104 developmental biology, Thistle

الوصف: The aim of this study was, firstly, to evaluate the phenol profile of thistle (Cirsium japonicum, CJ) by High performance liquid chromatography-electrospray ionization&ndash
mass spectrometry (HPLC&ndash
ESI&ndash
MS), dried by different methods (90 °
C hot-air, 70 °
C hot-air, shade-, and freeze-drying). Secondly, we aimed to evaluate the relationship between phenolic compounds content and antioxidant properties. CJ contained chlorogenic acid, linarin, and pectolinarin. Total phenolic contents of CJ significantly decreased under hot-air-drying condition, especially chlorogenic acid contents in CJ have been reduced by 85% and 60% for 90 °
C and 70 °
C hot-air-drying, respectively. We evaluated the protective effect on adrenal pheochromocytoma (PC12) cells and Caenorhabditis elegans using shade-dried CJ, which has the largest phenolic contents and the strongest antioxidant property. CJ-treated PC 12 cells dose-dependently exhibited the protective effects against reactive oxygen species (ROS), while cell viability increases, lactate dehydrogenase release decreases, and ROS formation decreases. Furthermore, CJ has also shown protection against ROS in C. elegans. Consequently, CJ contributed to lifespan extension under ROS stress without influencing the physiological growth.

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المؤلفون: Bartłomiej Perek, Mateusz Puślecki, Anna Komosa, Marek Grygier, Przemysław Niedzielski, Zbigniew Krasiński, Tatiana Mularek-Kubzdela, Barbara Poniedziałek, Marcin Misterski, Jolanta M. Siller-Matula, Mariola Ropacka-Lesiak, Anna Olasińska-Wiśniewska, Piotr Rzymski, Maciej Lesiak

المصدر: Journal of Clinical Medicine
Volume 8
Issue 9
Journal of Clinical Medicine, Vol 8, Iss 9, p 1364 (2019)

مصطلحات موضوعية: medicine.medical_specialty, Transcatheter aortic, genetic structures, Thiobarbituric acid, medicine.medical_treatment, Urology, lcsh:Medicine, 030204 cardiovascular system & hematology, medicine.disease_cause, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Aortic valve replacement, Valve replacement, Lactate dehydrogenase, medicine, TBARS, oxidative stress, 030212 general & internal medicine, transcatheter aortic valve implantation, business.industry, lcsh:R, General Medicine, medicine.disease, Antioxidant capacity, chemistry, total antioxidant capacity, business, Oxidative stress, surgical aortic valve replacement

الوصف: The objective of this study was to compare oxidative stress indices in 24 patients (mean ±
SD age 71 ±
13 years) undergoing surgical aortic valve replacement (SAVR) versus transcatheter aortic valve replacement (TAVR). Serum total antioxidant capacity (TAC), copper/zinc ratio (Cu/Zn), activity of lactate dehydrogenase (LDH), and thiobarbituric acid reactive substances (TBARS) were assessed at four different time-points: pre-procedure, immediately post-procedure, and one day and two days after the procedure. All oxidative stress parameters were comparable in both groups pre-procedure. TAC decreased significantly when assessed immediately after procedures in both groups (p <
0.001)
however, the magnitude of the reduction was more pronounced after SAVR (88% decrease from baseline: 1.8 ±
0.1 vs. 0.2 ±
0.03 mM) compared to TAVR procedures (53% decrease from baseline: 1.9 ±
0.1 vs. 1.0 ±
0.1 mM
p <
0.001). TAC returned to baseline two days after TAVR in all patients, but was still reduced by 55% two days after SAVR. In concordance, TBARS levels and Cu/Zn ratio increased significantly with maximum levels immediately after procedures in both groups (p <
0.001), but the magnitude of the increase was significantly higher in SAVR compared to TAVR (TBARS: 3.93 ±
0.61 µ
M vs. 1.25 ±
0.30 µ
M, p = 0.015
Cu/Zn ratio: 2.33 ±
0.11 vs. 1.80 ±
0.12
0.001). Two days after the procedure, TBARS levels and the Cu/Zn ratio returned to baseline after TAVR, with no full recovery after SAVR. TAVR is associated with a lesser redox imbalance and faster recovery of antioxidant capacity compared to SAVR.

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