Morphologic, Phylogenetic and Chemical Characterization of a Brackish Colonial Picocyanobacterium (Coelosphaeriaceae) with Bioactive Properties
| العنوان: | Morphologic, Phylogenetic and Chemical Characterization of a Brackish Colonial Picocyanobacterium (Coelosphaeriaceae) with Bioactive Properties |
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| المؤلفون: | Agata Błaszczyk, Jussi Meriluoto, Kerstin Häggqvist, Hanna Mazur-Marzec, Anna Toruńska-Sitarz |
| المصدر: | Toxins; Volume 8; Issue 4; Pages: 108 Toxins Toxins, Vol 8, Iss 4, p 108 (2016) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Cyanobacteria, DNA, Bacterial, Carboxypeptidases A, Health, Toxicology and Mutagenesis, anabaenopeptins, cyanopeptolins, enzyme inhibition, polyphasic approach, Snowella, Woronichinia, lcsh:Medicine, Biology, Toxicology, 01 natural sciences, Article, 03 medical and health sciences, Phylogenetics, Protein Phosphatase 1, RNA, Ribosomal, 16S, Botany, Chymotrypsin, Protein Phosphatase 2, Saline Waters, Phylogeny, Fragmentation (reproduction), Oligopeptide, Phylogenetic tree, Strain (chemistry), Pancreatic Elastase, 010604 marine biology & hydrobiology, lcsh:R, Thrombin, 16S ribosomal RNA, biology.organism_classification, 030104 developmental biology, Biochemistry, Cosmopolitan distribution, Oligopeptides |
| الوصف: | Despite their cosmopolitan distribution, knowledge on cyanobacteria in the family Coelosphaeriaceae is limited. In this study, a single species culture of a coelosphaeran cyanobacterium isolated from a brackish rock pool in the Baltic Sea was established. The strain was characterized by morphological features, partial 16S rRNA sequence and nonribosomal oligopeptide profile. The bioactivity of fractionated extracts against several serine proteases, as well as protein-serine/threonine phosphatases was studied. Phylogenetic analyses of the strain suggested a close relationship with Snowella litoralis, but its morphology resembled Woronichinia compacta. The controversial morphologic and phylogenetic results demonstrated remaining uncertainties regarding species division in this cyanobacteria family. Chemical analyses of the strain indicated production of nonribosomal oligopeptides. In fractionated extracts, masses and ion fragmentation spectra of seven possible anabaenopeptins were identified. Additionally, fragmentation spectra of cyanopeptolin-like peptides were collected in several of the fractions. The nonribosomal oligopeptide profile adds another potential identification criterion in future inter- and intraspecies comparisons of coelosphaeran cyanobacteria. The fractionated extracts showed significant activity against carboxypeptidase A and trypsin. Inhibition of these important metabolic enzymes might have impacts at the ecosystem level in aquatic habitats with high cyanobacteria densities. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6651 |
| DOI: | 10.3390/toxins8040108 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8f9504eba00935e685750a4b16f5804Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b8f9504eba00935e685750a4b16f5804 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726651 |
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| DOI: | 10.3390/toxins8040108 |