Is PTSD-Phenotype Associated with HPA-Axis Sensitivity?: The Endocannabinoid System in Modulating Stress Response in Rats
| العنوان: | Is PTSD-Phenotype Associated with HPA-Axis Sensitivity?: The Endocannabinoid System in Modulating Stress Response in Rats |
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| المؤلفون: | Hagit Cohen, Joseph Zohar, Doron Todder, Dor Danan |
| المصدر: | International Journal of Molecular Sciences Volume 22 Issue 12 International Journal of Molecular Sciences, Vol 22, Iss 6416, p 6416 (2021) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pituitary-Adrenal System, Hippocampal formation, Rats, Sprague-Dawley, Stress Disorders, Post-Traumatic, Basal (phylogenetics), chemistry.chemical_compound, 0302 clinical medicine, Cerebrospinal fluid, Corticosterone, anandamide, Biology (General), 2-arachidonoyl glycerol, Spectroscopy, Behavior, Animal, hypothalamus-pituitary-adrenal axis, pulsatility, General Medicine, Anandamide, Endocannabinoid system, Computer Science Applications, Chemistry, Phenotype, posttraumatic stress disorder, lipids (amino acids, peptides, and proteins), Hypothalamo-Hypophyseal System, medicine.medical_specialty, QH301-705.5, Urinary system, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Internal medicine, medicine, Animals, Secretion, Physical and Theoretical Chemistry, endocannabinoids, Molecular Biology, QD1-999, business.industry, animal model, corticosterone, Organic Chemistry, 030104 developmental biology, Endocrinology, chemistry, business, Stress, Psychological, 030217 neurology & neurosurgery |
| الوصف: | Endocannabinoids play a role in adaptation to stress and regulate the release of glucocorticoids in stressed and unstressed conditions. We recently found that basal corticosterone pulsatility may significantly impact the vulnerability for developing post-traumatic-stress-disorder (PTSD), suggesting that the endocannabinoid system may contribute to its development. To examine this, we exposed rats to predator scent stress (PSS). Behavioral reactions were recorded seven days post-PSS. Cerebrospinal fluid (CSF) was collected from anesthetized rats shortly after PSS exposure to determine the levels of 2-arachidonoyl glycerol (2-AG) and anandamide (AEA). To correlate between endocannabinoids and corticosterone levels, rats were placed in metabolic cages for urine collection. To assess the levels of endocannabinoids in specific brain regions, rats’ brains were harvested one day after behavioral analysis for staining and fluorescence quantification. Moreover, 2-AG was elevated in the CSF of PTSD-phenotype rats as compared with other groups and was inversely correlated with corticosterone urinary secretion. Eight days post-PSS exposure, hippocampal and hypothalamic 2-AG levels and hippocampal AEA levels were significantly more reduced in the PTSD-phenotype group compared to other groups. We posit that maladaptation to stress, which is propagated by an abnormal activation of endocannabinoids, mediates the subsequent stress-induced behavioral disruption, which, later, reduces neuronal the expression of endocannabinoids, contributing to PTSD symptomology. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms22126416 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::316709a3a73575dd509eb23fb73ef46eTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....316709a3a73575dd509eb23fb73ef46e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms22126416 |