Localized and Systemic Immune Responses against SARS-CoV-2 Following Mucosal Immunization
| العنوان: | Localized and Systemic Immune Responses against SARS-CoV-2 Following Mucosal Immunization |
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| المؤلفون: | Shaswath S Chandrasekar, Chungyi Hansen, M. Suresh, Adel M. Talaat, Rachel E Hildebrand, Yashdeep Phanse, Mostafa Hanafy, Chia-Wei Wu, Jorge E. Osorio |
| المصدر: | Vaccines Volume 9 Issue 2 Vaccines, Vol 9, Iss 132, p 132 (2021) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Modified vaccinia Ankara, medicine.medical_treatment, viruses, Immunology, Heterologous, lcsh:Medicine, Article, 03 medical and health sciences, 0302 clinical medicine, Immune system, Antigen, Immunity, Drug Discovery, intranasal vaccine, medicine, Pharmacology (medical), heterologous vaccine, Pharmacology, Heterologous vaccine, business.industry, SARS-CoV-2, lcsh:R, COVID-19, Virology, 030104 developmental biology, Infectious Diseases, Immunization, nanovaccine, business, Adjuvant, 030215 immunology |
| الوصف: | The rapid transmission of SARS-CoV-2 in the USA and worldwide necessitates the development of multiple vaccines to combat the COVID-19 global pandemic. Previously, we showed that a particulate adjuvant system, quil-A-loaded chitosan (QAC) nanoparticles, can elicit robust immunity combined with plasmid vaccines when used against avian coronavirus. Here, we report on the immune responses elicited by mucosal homologous plasmid and a heterologous immunization strategy using a plasmid vaccine and a Modified Vaccinia Ankara (MVA) expressing SARS-CoV-2 spike (S) and nucleocapsid (N) antigens. Only the heterologous intranasal immunization strategy elicited neutralizing antibodies against SARS-CoV-2 in serum and bronchoalveolar lavage of mice, suggesting a protective vaccine. The same prime/boost strategy led to the induction of type 1 and type 17 T-cell responses and polyfunctional T-cells expressing multiple type 1 cytokines (e.g., IFN-γ, TNFα, IL-2) in the lungs and spleens of vaccinated mice. In contrast, the plasmid homologous vaccine strategy led to the induction of local mono and polyfunctional T-cells secreting IFN-γ. Outcomes of this study support the potential of QAC-nano vaccines to elicit significant mucosal immune responses against respiratory coronaviruses. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-393X |
| DOI: | 10.3390/vaccines9020132 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8051269e09aaf5a4d5b8fda692fe211Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d8051269e09aaf5a4d5b8fda692fe211 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2076393X |
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| DOI: | 10.3390/vaccines9020132 |