FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia muciniphila
| العنوان: | FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia muciniphila |
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| المؤلفون: | Ann-Kathrin Kissmann, Barbara Spellerberg, Pengfei Xu, Heinz Fabian Raber, Hu Xing, Daniel Mayer, Nicholas Bodenberger, Tanja Weil, Dennis Kubiczek, Uwe Knippschild, Deena D’souza, Frank Rosenau |
| المساهمون: | European Union (EU), Horizon 2020 |
| المصدر: | International Journal of Molecular Sciences Volume 22 Issue 19 International Journal of Molecular Sciences, Vol 22, Iss 10425, p 10425 (2021) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DDC 540 / Chemistry & allied sciences, 02 engineering and technology, Feces, DDC 570 / Life sciences, Biology (General), Spectroscopy, 0303 health sciences, biology, Chemistry, SELEX Aptamer Technique, General Medicine, Aptamers, Nucleotide, 021001 nanoscience & nanotechnology, 3. Good health, Computer Science Applications, ddc:540, 0210 nano-technology, Akkermansia muciniphila, Aptamer, QH301-705.5, aptamers, Computational biology, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Human gut, Alzheimer Disease, ddc:570, FluCell-SELEX, Humans, Molecule, Physical and Theoretical Chemistry, QD1-999, Molecular Biology, 030304 developmental biology, Oligonucleotide, Organic Chemistry, Akkermansia, biology.organism_classification, biosensors, Gastrointestinal Microbiome, Biosensors, Biosensor, Systematic evolution of ligands by exponential enrichment, Bacteria |
| الوصف: | Based on their unique properties, oligonucleotide aptamers have been named a gift of biological chemistry to life science. We report the development of DNA aptamers as the first high-affinity binding molecules available for fast and rapid labeling of the human gut bacterium Akkermansia muciniphila with a certain impact on Alzheimer´s disease. Fast and reliable analyses of the composition of microbiomes is an emerging field in microbiology. We describe the molecular evolution and biochemical characterization of a specific aptamer library by a FluCell-SELEX and the characterization of specific molecules from the library by bioinformatics. The aptamer AKK13.1 exerted universal applicability in different analysis techniques in modern microbiology, including fluorimetry, confocal laser scanning microscopy and flow cytometry. It was also functional as a specific binding entity hybridized to anchor primers chemically coupled via acrydite-modification to the surface of a polyacrylamide-hydrogel, which can be prototypically used for the construction of affinity surfaces in sensor chips. Together, the performance and methodological flexibility of the aptamers presented here may open new routes not only to develop novel Akkermansia-specific assays for clinical microbiology and the analyses of human stool samples but may also be an excellent starting point for the construction of novel electronic biosensors. publishedVersion |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms221910425 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3015933ed409c5959ed7b5229585636Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a3015933ed409c5959ed7b5229585636 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms221910425 |