مورد إلكتروني
Temperature and trapping characterization of an acoustic trap with miniaturized integrated transducers - towards in-trap temperature regulation
| العنوان: | Temperature and trapping characterization of an acoustic trap with miniaturized integrated transducers - towards in-trap temperature regulation |
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| بيانات النشر: | Mikrosystemteknik 2013 |
| تفاصيل مُضافة: | Johansson, Linda Evander, Mikael Lilliehorn, Tobias Almqvist, Monica Nilsson, Johan Laurell, Thomas Johansson, Stefan |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | An acoustic trap with miniaturized integrated transducers (MITs) for applications in non-contact trapping of cells or particles in a microfluidic channel was characterized by measuring the temperature increase and trapping strength. The fluid temperature was measured by the fluorescent response of Rhodamine B in the microchannel. The trapping strength was measured by the area of a trapped particle cluster counter-balanced by the hydrodynamic force. One of the main objectives was to obtain quantitative values of the temperature in the fluidic channel to ensure safe handling of cells and proteins. Another objective was to evaluate the trapping-to-temperature efficiency for the trap as a function of drive frequency. Thirdly, trapping-to-temperature efficiency data enables identifying frequencies and voltage values to use for in-trap temperature regulation. It is envisioned that operation with only in-trap temperature regulation enables the realization of small, simple and fast temperature-controlled trap systems. The significance of potential gradients at the trap edges due to the finite size of the miniaturized transducers for the operation was emphasized and expressed analytically. The influence of the acoustic near field was evaluated in FEM-simulation and compared with a more ideal 1D standing wave. The working principle of the trap was examined by comparing measurements of impedance, temperature increase and trapping strength with impedance transfer calculations of fluid-reflector resonances and frequencies of high reflectance at the fluid-reflector boundary. The temperature increase was found to be moderate, 7 degrees C for a high trapping strength, at a fluid flow of 0.5 mm s(-1) for the optimal driving frequency. A fast temperature response with a fall time of 8 s and a rise time of 11 s was observed. The results emphasize the importance of selecting the proper drive frequency for long term handling of cells, as opposed to the more pragmatic way of selecting th |
| مصطلحات الفهرس: | Acoustic trapping, Temperature analysis, Micro total analysis system, FEM simulation, Temperature regulation, Engineering and Technology, Teknik och teknologier, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1016.j.ultras.2013.01.010 |
| URL: | Ultrasonics, 0041-624X, 2013, 53:5, s. 1020-1032 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:uu-200031 doi:10.1016/j.ultras.2013.01.010 ISI:000317184400013 1235051315 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1235051315 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1016.j.ultras.2013.01.010 |
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